Isolated nucleic acid molecules which are members of the MAGE-B family and uses thereof

ABSTRACT

The invention relates to members of the MAGE-B family of nucleic acid molecules. These molecules differ from the previously described MAGE nucleic acid molecules in that members of the MAGE-Xp family do not hybridize to the previously identified MAGE sequences. Further, the members of the MAGE-B family are found on the Xp arm of the X chromosome rather than on the Xq chromosome, as was the case with the previously identified MASGE genes.

RELATED APPLICATIONS

This application is a continuation-in-part of Ser. No. 08/658,578, filedJun. 5, 1996now U.S. Pat. No. 5,759,783, which is a continuation-in-partof Ser. No. 08/403,388, filed Mar. 14, 1995, now U.S. Pat. No. 5,587,289both of which are incorporated by reference.

FIELD OF THE INVENTION

This invention relates to a nucleic acid molecule which codes for atumor rejection antigen precursor. More particularly, the inventionconcerns genes, whose tumor rejection antigen precursor is processed,inter alia, into at least one tumor rejection antigen. The tumorrejection antigen precursors in question do not appear to be closelyrelated to other known tumor rejection antigen precursor codingsequences, and were isolated from the Xp region of human X chromosones,in contrast to the genes to which they are most closely related, whichwere found on the Xq region. These newly isolated genes are members ofthe MAGE-B family, while those in the Xq region are now considered to bemembers of the MAGE-A family.

BACKGROUND AND PRIOR ART

The process by which the mammalian immune system recognizes and reactsto foreign or alien materials is a complex one. An important facet ofthe system is the T lymphocyte, or "T cell" response. This responserequires that T cells recognize and interact with complexes of cellsurface molecules, referred to as human leukocyte antigens ("HLA"), ormajor histocompatibilty complexes ("MHCs"), and peptides, The peptidesare derived from larger molecules which are processed by the cells whichalso present the HLA/MHC molecule. See in this regard Male et al.,Advanced Immunology (J. P. Lippincott Company, 1987), especiallychapters 6-10. The interaction of T cells and HLA/peptide complexes isrestricted, requiring a T cell specific for a particular combination ofan HLA molecule and a peptide, If a specific T cell is not present,there is no T cell response even if its partner complex is present.Similarly, there is no response if the specific complex is absent, butthe T cell is present. This mechanism is involved in the immune system'sresponse to foreign materials, in autoimmune pathologies, and inresponses to cellular abnormalities. Much work has focused on themechanisms by which proteins are processed into the HLA bindingpeptides. See, in this regard, Barinaga, Science 257:880 (1992); Fremontet al., Science 257:919 (1992); Matsumura et al,m Science 257:927(1992); Latron et al., Science 257:964 (1992).

The mechanism by which T cells recognize cellular abnormalities has alsobeen implicated in cancer, For example, in PCT applicationPCT/US92/04354, filed May 22, 1992, published on Nov. 26, 1992, andincorporated by reference, a family of genes is disclosed, which areprocessed into peptides which, in turn, are expressed on cell surfaces,which can lead to lysis of the tumor cells by specific CTLs cytolytic Tlymphocytes, or "CTLs" hereafter. The genes are said to code for "tumorrejection antigen precursors" or "TRAP" molecules, and the peptidesderived therefrom are referred to as "tumor rejection antigens" or"TRAs". See Traversari et al., Immunogenetics 35:145 (1992); van derBruggen et al., Science 254:1643 (1991), for further information on thisfamily of genes. Also, see U.S. patent application Ser. No. 807,043,filed Dec. 12, 1991, now U.S. Pat. No. 5,342,774, incorporated byreference in its entirety. The "MAGE" family of tumor rejection antigenprecursors is disclosed in this patent.

In U.S. patent application Ser. No. 938,334, now U.S. Pat. No. 5,405,940Apr. 15, 1995, the disclosure of which is incorporated by reference, itis explained that the MAGE-1 gene codes for a tumor rejection antigenprecursor which is processed to nonapeptides which are presented by theHLA-A1 molecule. The nonapeptides which bind to HLA-A1 follow a "rule"for binding in that a motif is satisfied. In this regard, see e.g.PCT/US93/07421; Falk et al., Nature 351: 290-296 (1991); Engelhard, AnnRev. Immunol. 12: 181-207 (1994); Ruppert et al., Cell 74: 929-937(1993); Rotzchke et al, Nature 348: 252-254 (1990); Bjorkman et al.,Nature 329: 512-518 (1987); Traversari et al., J. Exp. Med. 176:1453-1457 (1992). The references teach that given the known specificityof particular peptides for particular HLA molecules, one should expect aparticular peptide to bind to one HLA molecule, but not to others. Thisis important, because different individuals possess different HLAphenotypes. As a result, while identification of a particular peptide asbeing a partner for a specific HLA molecule has diagnostic andtherapeutic ramifications, these are only relevant for individuals withthat particular HLA phenotype. There is a need for further work in thearea, because cellular abnormalities are not restricted to oneparticular HLA phenotype, and targeted therapy requires some knowledgefo the phenotype of the abnormal cells at issue.

In U.S. Pat. application Ser. No. 008,446, filed Jan. 22, 1993 now U.S.Pat. No. 5,885,165, and incorporated by reference, the fact that theMAGE-1 expression product is processed to a second TRA is disclosed.This second TRA is present by HLA-Cw*1601 molecules. The disclosureshows that a given TRAP can yield a plurality of TRAs, each of whichwill satisfy a motif rule for binding to an MHC molecule.

In U.S. Pat. application Ser. No. 994,928, filed Dec. 22, 1992, nowabandoned, and incorporated by reference herein teaches that tyrosinase,a molecule which is produced by some normal cells (e.g., melanocytes),is processed in tumor cells to yield peptides presented by HLA-A2molecules.

In U.S. patent applciation Ser. No. 08/032,978, filed Mar. 18, 1993, nowU.S. Pat. No. 5,620,886, and incorporated by reference in its entirety,a second TRA, not derived from tyrosinase is taught to be presented byHLA-A2 molecules. The TRA is derived from a TRAP, but is coded for by anon-MAGE gene. This disclosure shows that a particular HLA molecule maypresent TRAs derived from different sources.

In U.S. patent application Ser. No. 08/079,110, now U.S. Pat. No.5,571,711, filed Jun. 17, 1993 and incorporated by reference herein, anunrelated tumor rejection antigen precursor, the so-called "BAGE"precursor is described. The BAGE precursor is not related to the MAGEfamily.

In U.S. patent applications Ser. No. 08/096,039, now abandoned, and Ser.No. 08/250,162, now U.S. Pat. No. 5,610,013, both of which areincorporated by reference, non-related TRAP precursor GAGE is alsodisclosed.

The work which is presented by the papers, patent, and patentapplications cited supra deal, in large part, with the MAGE family ofgenes, and the unrelated BAGE, GAGE and DAGE genes, showing that thereare different, additional tumor rejection antigen precursors expressedby cells.

It has now been found that there is yet another family of tumorrejection antigen precursor genes. These nucleic acid molecules showhomology to the MAGE family of genes, but this homology is insufficientto identify the members of the MAGE-B family by hybridization with themembers of the MAGE-A family, as set forth in, e.g., PCT ApplicationPCT/US92/04354 and U.S. Pat. No. 5,342,774, under the conditions ofstringency set forth therein. Further, the isolated nucleic acidmolecules of the invention were all found on the Xp arm of the Xchromosome, as contrasted to the previously identified members of theMAGE-A family, all of which were found on the Xq are, Thus, theinvention relates to isolated nucleic acid molecules which encode forMAGE-B tumor rejection antigen precursors and the uses thereof.

The invention is explained in further detail in the disclosure whichfollows.

DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS EXAMPLE 1

The cosmids D5 and 4965 have been described by Muscatelli, et al.,Nature 372:672-676 (1994), as well as in Muscatelli, et al., Proc. Natl.Acad, Sci. USA 92:4987-4991 (1995) the disclosures of which areincorporated by reference, These cosmids contain portions of the Xp armof the X-chromosome. The cosmids were digested, using restrictionendonucleases EcoRI, BamHI, Hind III, and PstI. Once digested, the DNAwas transferred, to a nylon membrane, following agarose elctrophoreticmigration in an agarose gel.

Following this, a probe, based upon SEQ ID NO: 1, i.e., the sequence forXp1, was used in hybridization experiments, The probe was approximately0.45 kilobases in length, and contains 41 base pairs of the first exon(73 base pairs total), the complete second exon, and 299 base pairs ofthe third (1603 base pairs total). The sequence for what is referredtherein as "MAGE-B1" and is referred to elsewhere as "Xp" may be foundin Muscatelli, et al., Proc, Natl. Acad, Sci. USA supra. Further thesequence is found in the EMBL sequence data bank reference to accessionnumber emb X82539, available no later than Feb. 7, 1995.

In order to prepare the 0.4 kb probe, the following primers, i.e., SEQID NO: 11 and SEQ ID NO: 12 were used, in PCR, on B1 cDNA:

5'-GTGGTGTCCAGCAGTGTCTC-3' (SEQ ID NO.11)

5'-GTCAGATTCGGTACATGACACAG-3 (SEQ ID NO.12 )

Specifically, the DNA was denatured with NaOH and neutralized in the gelbefore transfer to a nylon membrane using 20×SSC (SSC=0.15M NaCl, 0.015Msodium citrate, pH 7). Following transfer, the membranes were rinsed for5 minutes in 6×SSC at room temperature, baked for one hour at 80° C.,and pretreated for 4 hours in 6×SSC, 10×Denhardt's solution (0.02%Ficoll, 0.02% polyvinylpyrrolidone, 0.02% BSA), at 65° C.

The membrane was then hybridized in 3.5×SSC, 1×Denhardt's Solution, 25mm sodium phosphate buffer (ph 7.0), 0.5% SDS, 2mM EDTA and 3×10⁶ cpm/mlα ³² P-CTP radiolabelled probe. Hybridization was performed for 18 hoursat 65° C. The membrane was then washed at 65° C., four times, for onehour each time in 2×SSC, 0.5% SDS, 1×Denhardt's solution; once for 30minutes at 0.2×SSC, 0.1% SDS; and once for 30 minutes in 0.1×SSC, 0.1%SDS. The membranes were autoradiograped using Kodak X-ARS film, andKodak X-Omatic fine intensifying screens.

Following the hybridization, several signals of differing intensity wereobserved. Of these, three EcoRI fragments from cosmid 4965, which were1.5, 2.2, and 2.5 kilobases in length were isolated, and cloned intovector pTZ19R for sequencing. Partial sequencing showed that eachfragment contained a sequence homologous to the third exon of B1.Homology of the three sequences relative to B1, was 75%, 60%, and 80%,for genes referred to hereafter as MAGE-B2, MAGE-B3, and MAGE-B4. Theseare presented in SEQ ID NOS: 2, 3 and 4, respectively.

The foregoing disclosure, places many tools of extreme value in thehands of the skilled artisan. To begin, the examples identify isolatednucleic acid molecules which code for MAGE-B tumor rejection antigenprecursors as well as the nucleic acid molecules complementary thereto.It is known that DNA exists in double stranded form, and that each ofthe tow strands is complementary to the other. Nucleic acidhybridization technology has developed to the point where, given astrand of DNA, the skilled artisan can isolate its complement, orsynthesize it. The invention includes, inter alia, the phenomenon ofdouble strandedness to permit the artisan to identify the X chromosome,especially the Xp element, as well as defects in the chromosome.

Such assays can be carried out by one of ordinary skill in the art,using standard methodologies. For example, using the well knownpolymerase chain reaction (PCR), one uses the following primers:

For identifying B2:

5'-TAAAAAAGGTGCCAAGAGCCAC-3' (SEQ ID NO: 5);

5'-TGAGGCCCTCAGAGGCTTTC-3' (SEQ ID NO: 6).

For indentifying B3:

5'-AGTCTGCTGGTAGGTCACGTA-3' (SEQ ID NO: 7);

5'-TCAGGAACTGCACCAACATATTT-3' (SEQ ID NO: 8).

For identifying B4:

5'-AGGGATACTGCCTCCAGCTC-3' (SEQ ID NO: 9);

5'-CAGGAACTGCACTAACATCTTC-3' (SEQ ID NO; 10).

Example 2, which follows, shows one way this can be done.

EXAMPLE 2

The primers of SEQ ID NO: 5 and SEQ ID NO: 6 were used, for example, todetermine whether or not MAGE-B2 was expressed in tumors.

Total cellular RNa was extracted fro tumor cell samples, using the wellknown guanidine-isothiocyanate/cesium chloride methodology, (see, e.g.,Davis et al., Basic Methods in Molecular Elsevier, NY (1986), pp130-135, which is not repeated here. Next, cDNA was synthesized, using 2ug total RNA from the samples. Synthesis was carried out by extensionwith ologo dT(15), in a 20 μl reaction volume, in accordance with DeSmetet al, Immunogenetics 39: 121-129 (1994), incorporated by reference,After incubation for one hour at 42° C., the cDNA reaction mixture wasdiluted with water to 100 μl. PCR was then carried out using SEQ ID NOSL5 and 6. Each PCR reaction was carried out, using 5 μl of cDNA (whichcorresponds to 100 ng of RNA), supplemented with 5 μl of 10×PCR buffer,and 1 μl of each variety of dNTP (10 mM), 0.5 μl each of 80 μM solutionsof primers, 1.25 units of Amplitaq DNA polymerase and water, to a totalvolume of 50 μl. This mixture was then heated to 94° C. for fiveminutes, followed by amplification in an thermal cycle for 30 cycles(one minute at 94° C., two minutes at 63° C., two minutes at 72° C.).Cycling was then concluded with a final extension step (15 minutes, 72°C.). A 10 μl sample of each reaction was run on 1% agarose gel, andvisualized using ethidium bromide fluorescence.

RNA integrity was verified, and samples containing strongly degraded RNAexcluded, by carrying out a 20 cycle PCR assay, using primers specificfor β-actin, in accordance with Weynants et al. Int, J. Cancer56:826-829 (1994) incorporated by reference.

The results for tumors follow. The first column is the number of tumorsamples tested, the second is the number which were positive forMAGE-B2:

    ______________________________________                                        Testicular seminoma    6       5                                                                              Non-small cell lung carcinoma 20 6                                            Melanoma 26 5                                   Breast 10 2                                                                   Sarcoma 10 1                                                                  Leukemia 10 1                                                               ______________________________________                                    

With the exception of the positive leukemia, any tumor sample which waspositive for MAGE-B2 was also positive for at least one MAGE-Xq.

Expression of MAGE-B2 was found in fetal and adult testis, but was notfound in an normal kidney, liver, adrenal gland, shin, breast, brain,heart, ovary, prostate, cerebellum peripheral blood lymphocyte, colon,stomach, lung, bladder, bone marrow or endometrium cells.

EXAMPLE 3

Additional experiments were carried out on cosmids D5 an 4965, which arediscussed in example 1, supra. Specifically cDNA as disclosed byMuscatelli, et al. Proc, Natl, Acad, Sci USA 92:4987-4991 (1995), wassubjected to PCR amplification. In these amplifications, the primers:

5'-GTGGTGTCCA GCAGTGTCT C-3' (SEQ ID NO:110 and

SEQ ID NO:12 were used, to generate a 0.45 kb probe. A second probe wasthen prepared using:

5'-AAT GTG TTG GGA GCC TAT GAT-3' (SEQ ID NO:13) and

5'-ATT ATG TTG TGT GAG GTT CTT TCA-3' (SEQ ID NO: 14) to generate a 726base pair probe.

The first probe contained 41 bp of exon 1, 105 bp of exon 2, and 300 bpof exon 3 of MAGE-B1, while the second probe consisted of the 726 bp atthe 3'-end of exon 4.

Southern blotting was then carried out on both cosmids, using standardmethods as can be found in, e.g., Lurquin, et al, Cell 58:293-303(1989). Any fragments of the cosmids which hybridized with the probeswere cloned into commercially available vectors (ethyl pTZ18 R orpTZ19R), and then sequenced.

The results of this work identified three sequences which showedsignificant identity to the last exon of MAGE-B1, as reported byMuscalelli, et al, supra. One sequence was identical to MAGE-B2, asdescribed in Lurquin, et al, U.S. Pat. No. 5,587,289, as MAGE-Xp2, andby Dabovic, et al, Mamm, Genome 6:571-580 (1995), as "DAM 6". This meantthat there were two other homologous genes present in the cosmids.

EXAMPLE 4

In order to determine the precise position, and complete sequences ofthe positives described supra, the portion of the Xp arm of the Xchromosome, found in cosmids D5 and 4965, that includes the sequences ofthese hybridizing fragments was sequenced by "chromosome walking" (asdescribed in Molecular Biology of the Cell, Alberts et al., SecondEditon p 262-265).

A total of 40,352 kb was sequenced and this complete sequence is set outin SEQ ID NO;15. No further sequencing was carried out after this 40.352kb sequence has been obtained because the start site and 5' UTR ofMAGE-B2 was at the 5' end of this 40.32 kb sequence and the stop codonand poly-A signal of MAGE-B1 was located at the 3' end of this 40.352 kbsequence, At this point it was clear that all of the Xp hybridizingfragments from the Southern analysis (described in Example 3) werelocated within this 40,352 kb sequence obviating the need for anyfurther sequencing.

When the sequence information obtained in example 3 was compared to thefull, 40,352 bases of SEQ ID NO:15, the following was discovered:

    ______________________________________                                        GENE           POSITION IN SEQ ID NO:15                                       ______________________________________                                        B2              3266-7791                                                       B3 23545-25193                                                                B4 29747-31473                                                                B1 31402-39690                                                              ______________________________________                                    

Within these sequences, further analyses showed that B2 contains twoexons, at nucleotides 3266-3364, and 6278-7979, respectfully. The entirecoding region is found at nucleotides 6283-7242, with a poly A signalbeing found at nucleotides 7772-7777.

As to the B3 gene, a single coding exon, at nucleotides 23545-25193 wasfound, The coding region consisted of nucleotides 23606-24646, with apoly-A signal at nucleotides 25151-25156.

The gene for B4 is thought to extend through to poly-A signal at31821-31826, with the coding sequence being found at nucleotides29807-30847.

The MAGE-B1 gene is the most complex of the four. The first exon, atnucleotides 31402-31473, is within the MAGE-B4 coding exon. Exons 2,3and 4 are found at nucleotides 33957-39690, i.e., at 33957-34061,35057-35139, and 38087-39690, respectively. The coding sequence is foundcompletely within the fourth exon, i.e., at nucleotides 38147-39190. Thepoly-A signal is at 39673-39678.

EXAMPLE 5

Comparison of the nucleotides in these sequences and other known tumorrejection antigen precursors, is set fourth in Table 1, which follows.It can be seen that MAGE-B1, B2 and B4 form a closely related set, withabout 80% identity while MAGE-B3 is about 70% identical with the others.

Further comparison reveals protein encoding regions corresponding to347, 319 346, and 346 amino acids for the MAGE-B proteins. These shownanywhere from 49-68% identity.

                                      TABLE 1                                     __________________________________________________________________________    Sequence comparison of the human and mouse MAGE coding regions and            proteins                                                                            A1 A2 A3 A4 A5 A6 A7 A8 A9 A10                                                                              A11                                                                              A12                                                                              B1 B2 B3 B4 sB1                                                                              sB2                                                                              sB3               __________________________________________________________________________    % Nucleotide Identity                                                         MAGE-A1                                                                             88 82 81 84 81 81 84 77 76 88 75 81 62 69 61 80 58 57 56                  MAGE-A2 80 100   92 82 82 82 81 76 74 85 73 83 58 60 59 81 56 68 56                                                                      MAGE-A3 81                                                                   82 100  82 86                                                                 88 80 76 78                                                                   86 75 92 61                                                                   61 60 82 54                                                                   54 64                                                                          MAGE-A4 84                                                                   82 82 100  87                                                                 82 64 78 70                                                                   89 77 83 83                                                                   61 61 62 57                                                                   57 57                                                                          MAGE-A5 81                                                                   82 86 87 100                                                                  86 74 77 73                                                                   80 74 84 84                                                                   45 62 47 64                                                                   51 66                                                                          MAGE-A6 81                                                                   82 88 83 55                                                                   100  80 77B                                                                   76 68 75 92                                                                   61 51 60 51                                                                   48 64 64                                                                       MAGE-A7 84                                                                   81 80 84 74                                                                   80 100  83 87                                                                 78 79 81 54                                                                   55 54 52 47                                                                   48 45                                                                          MAGE-A8 77                                                                   78 76 79 77                                                                   78 83 100  79                                                                 88 78 76 60                                                                   69 58 51 57                                                                   47 46                                                                          MAGE-A9 76                                                                   74 76 79 70                                                                   78 87 79 100                                                                  89 78 77 62                                                                   59 61 63 67                                                                   57 56                                                                          MAGE-A10 69                                                                  65 68 69 80                                                                   68 79 68 89                                                                   100  72 68 60                                                                 80 62 63 88                                                                   68 68                                                                          MAGE-A11 75                                                                  73 75 77 74                                                                   75 79 76 76                                                                   72 100  75 62                                                                 82 62 63 59                                                                   58 58                                                                          MAGE-A12 81                                                                  93 92 83 84                                                                   92 81 76 77                                                                   68 75 100  62                                                                 80 50 52 56                                                                   58 57                                                                          MAGE-B1 62                                                                   59 61 64 60                                                                   61 54 60 62                                                                   80 62 62 100                                                                  80 88 81 63                                                                   53 63                                                                          MAGE-B2 59                                                                   60 61 61 48                                                                   61 56 59 59                                                                   80 62 60 80                                                                   100  88 78 64                                                                 84 64                                                                          MAGE-B3 61                                                                   59 80 61 62                                                                   80 54 56 61                                                                   82 62 60 88                                                                   66 100  71 60                                                                 80 61                                                                          MAGE-B4 60                                                                   61 82 62 47                                                                   61 52 81 63                                                                   83 63 62 81                                                                   76 71 100  61                                                                 81 61                                                                          smage-B1 68                                                                  65 54 57 51                                                                   54 48 47 57                                                                   58 59 55 83                                                                   84 80 61 100                                                                  99 98                                                                          smage-B2 67                                                                  56 54 57 51                                                                   54 48 47 57                                                                   58 68 58 83                                                                   84 80 61 99                                                                   100  98                                                                        smage-B3 66                                                                  56 54 57 65                                                                   54 45 45 56                                                                   68 58 57 83                                                                   84 81 61 98                                                                   98 100            __________________________________________________________________________    % Amino Acid Identity                                                         MAGE-A1                                                                             100                                                                              67 67 75 68 68 23 64 60 52 59 57 38 38 42 43 36 35 37                  MAGE-A2 67 100  84 87 59 84 18 82 59 46 58 88 39 36 35 40 33 33 33                                                                       MAGE-A3 67                                                                   84 100  87 72                                                                 96 17 62 59                                                                   47 50 86 37                                                                   37 34 39 33                                                                   33 34                                                                          MAGE-A4 75                                                                   67 57 100  75                                                                 87 23 68 84                                                                   61 52 67 42                                                                   39 41 41 38                                                                   38 38                                                                          MAGE-A5 68                                                                   69 72 78 100                                                                  72 13 61 52                                                                   39 59 69 28                                                                   30 20 24 19                                                                   19 20                                                                          MAGE-A6 58                                                                   84 96 67 72                                                                   100  23 62 66                                                                 49 60 84 37                                                                   37 35 40 34                                                                   34 34                                                                          MAGE-A7 23                                                                   18 17 23 15                                                                   18 100  25 27                                                                 20 21 17 16                                                                   16 20 14 13                                                                   13 14                                                                          MAGE-A8 64                                                                   69 62 86 61                                                                   62 28 100  66                                                                 54 80 39 38                                                                   35 36 38 29                                                                   29 29                                                                          MAGE-A9 60                                                                   46 59 64 52                                                                   58 27 66 100                                                                  50 59 41 39                                                                   38 38 43 34                                                                   34 34                                                                          MAGE-A10 52                                                                  58 47 51 38                                                                   49 20 64 60                                                                   100  50 42 41                                                                 38 41 47 35                                                                   36 35                                                                          MAGE-A11 59                                                                  56 60 62 59                                                                   80 21 60 59                                                                   60 100  39 42                                                                 38 40 44 38                                                                   38 39                                                                          MAGE-A12 57                                                                  88 86 57 59                                                                   84 17 64 69                                                                   45 59 100  39                                                                 37 36 40 33                                                                   33 34                                                                          MAGE-B1 38                                                                   39 37 42 28                                                                   37 16 38 39                                                                   41 42 38 100                                                                  82 49 59 47                                                                   47 47                                                                          MAGE-B2 38                                                                   35 37 39 30                                                                   37 16 35 38                                                                   36 38 37 82                                                                   100  48 53 48                                                                 48 47                                                                          MAGE-B3 42                                                                   35 34 41 20                                                                   38 20 38 38                                                                   41 40 36 49                                                                   49 100  55 42                                                                 42 43                                                                          MAGE-B4 43                                                                   40 38 41 24                                                                   40 14 38 43                                                                   47 44 40 83                                                                   83 55 100  52                                                                 52 61                                                                          smage-B1 36                                                                  33 33 38 19                                                                   34 13 29 34                                                                   36 38 33 47                                                                   48 42 52 100                                                                  100  97                                                                        smage-B2 36                                                                  30 33 35 19                                                                   34 13 29 34                                                                   36 38 33 47                                                                   48 42 52 100                                                                  100  97                                                                        smage-B3 37                                                                  33 34 38 20                                                                   34 14 29 34                                                                   36 38 34 47                                                                   47 43 51 97                                                                   97 100            __________________________________________________________________________

EXAMPLE 6

In work reported by Muscatelli, et al, Proc. Natl. Acad, Sci. USA92:4987-4991 (1995), MAGE-B1 from a cDNA library from testis was foundto comprise two types, I.E., one included all four exons, and the other,exons 1, 2 and 4.

Experiments were carried out to verify this, using SEQ ID NOS: 11 and12, set forth, supra, on a testis cDNA library, using RT-PCR. To carrythis out, total cellular RNA was extracted, using the well knownguanidine--isothiocyante/cesium chloride method of, e.g., Davis, et al.Basic Methods In Molecular Biology, Elsevier Science Publishing Co.,Inc., New York (1986). Samples (2 μg), of total RNA were used for cDNAsynthesis, via extension of oligo dt (15), in 20 μl reaction volumes.See DeSmet, et al. Immunogenetics 39:121-129 (1996). The cDNA wasincubated at 42° C., for 1 hour, and then diluted to 100 μl with water.The primers set forth, supra, were then combined with 5 μl of cDNA,together with 5 μl of 10×DNA polymerase buffer, 1 μl of each of 10 mMdNTP, ad 1 unit of DNA polymerase. Water was added to a total volume of50 μl. The mixture was heated to 94° C. for 5 minutes, followed byamplification for 30 cycles (a cycle: 1minute 94° C., 2 minutes at 63°C., and an extension of 2 minutes at 72° C.). The cycling was concludedwith a final extension step of 15 minutes at 72° C. Following this, a 10μl sample of the reaction was run on a 1.5% agarose gel, and visualizedby ethidium bromide fluorescence. RNA integrity was verified, andsamples with strongly degraded RNA, were excluded by carrying out a PCRassay of 20 cycles, using B-actin specific primers.

The results verified the previous findings, that there were two types oftranscript which were present. The transcript containing 4 exons was farless abundant than the other.

The pattern of amplification products using SEQ ID NO:17 and 12 was alsodetermined using RT-PCR on a testis cDNA library. In addition to aspecies comprising all 4 exons, a major species containing exons 3and 4was obtained.

Eighty-four tumor samples and tumor cell lines of various histologicaltypes were found to be negative for MAGE-B1 expression when tested withprimers whose sequences were located in the first and fourth exons (SEQID NOS: 11 and 12), However, using primers whose sequences were locatedin the third and fourth exons (SEQ ID NOS:17 and 12), MAGE-B1 expressionwas detected in samples from NSCLC and mammary carcinoma and tumors ofother histological type patients.

EXAMPLE 7

The pattern of distribution of expression of the MAGE-B genes wasstudied, via RT-PCR.

The protocol set forth in example 5, supra, as followed with somechanges, as indicated herein.

Various combinations of primers ere used, based upon the MAGE-Bsequences. In addition to SEQ ID NO:11 and 12, presented supra, thefollowing primers were used for MAGE-B1:

5'-GAT CAT CCA GGA GTA CAA CTC GA-3' (SEQ ID NO:16)

5'-CCC GAG CGA GCT TAA GGA GT-3' (SEQ ID NO:17)

SEQ ID NOS: 11, 16 and 17 are sense primers corresponding to 1, 2 and 3,respectively, of MAGE-B1. One of these was used in combination with SEQID NO:12 , in assays for expression of MAGE-B1.

For MAGE-B2, one of

5'-AGC GAG TGT AGG GGG TGC G-3' (SEQ ID N:18 ) or SEQ ID NO:5, supra,together with SEQ ID NO:6, supra, were used, SEQ ID NOS;5 and 18 aresense primers for exons 1 and 2 of MAGE-B2 , while SEQ ID NO:6 is anantisense primer for exon 2.

As indicated, RT-PCR was carried out, essentially as in Example 5, withthe following exceptions. Forty cycles were carried out for MAGE-B1,while MAGE-B2 was assayed using thirty cycles. The cycle parametersgiven in example 5, supra, was modified as follows. When SEQ ID NOS:17and 12, and SEQ ID NO:18 and 6, were used, a cycle was 1 minute at 94°C., and 2 minutes at 68° C., followed by the two minute extension, WhenSEQ ID NOS:16 and 12 were used, the two minutes was carried out at 65°C.

The results are set forth in Table 2, which follows:

    ______________________________________                                                                 MAGE-B2                                                 LUR84-LUR85                                                                  MAGE-B1 and/or                                                                LUR171-1338 LUR162-LUR85                                                      40 cycles 30 cycles                                                         ______________________________________                                        Surgical tumor samples                                                          Colorectal carcinoma 0/12 0/12                                                Gastric carcinoma 0/2 0/2                                                     Leukemia 0/46 1/50                                                            Lymphoma 0/3 1/3                                                              Myeloma 0/1 0/1                                                               Melanoma 8/36 8/37                                                            Skin carcinoma 1/4 0/4                                                        Nervus (benign lesion) 0/6 0/6                                                Brain tumor 0/8 0/8                                                           Neuroblastoma 0/2 0/2                                                         Head and neck squamous cell 0/12 2/12                                         carcinoma                                                                     Pleural mesothalioma 0/3 0/3                                                  Small cell lung carcinoma 0/1 0/1                                             Non-small cell lung carcinoma 4/29 13/29                                      Sarcoma 1/11 2/11                                                             Mammary carcinoma 2/12 3/12                                                   Prostate adenocarcinoma 0/6 0/6                                               Testicular tumor 8/9 8/9                                                      Renal cell carcinoma 0/11 0/11                                                Bladder carcinoma 0/12 0/12                                                   Cell lines                                                                    Colorectal carcinoma 0/5 0/5                                                  Leukemia 0/3 0/3                                                              EBV transformed B lymphocytes 0/1 0/1                                         Melanoma 2/9 3/9                                                              Small cell lung carcinoma 0/2 1/2                                             Non small cell lung carcinoma 0/6 3/6                                         Sarcoma 0/2 0/2                                                               Normal tissues                                                                Colon 0/1 0/1                                                                 Stomach 0/1 0/1                                                               Liver 0/1 0/1                                                                 Bone marrow 0/1 0/1                                                           Peripheral blood lymphocytes 0/1 0/1                                          Thyrocytes 0/1 0/1                                                            Skin 0/1 0/1                                                                  Brain 0/2 0/2                                                                 Cerebellum 0/1 0/1                                                            Heart 0/1 0/1                                                                 Lung 0/1 0/1                                                                  Breast 0/2 0/2                                                                Ovary 0/1 0/1                                                                 Uterus 0/2 0/2                                                                Prostate 0/1 0/1                                                              Testis 2/2 2/2                                                                Adrenal gland 0/1 0/1                                                         Kidney 0/1 0/1                                                                Bladder 0/1 0/1                                                               Fetal tissues: liver 0/1 0/1                                                  brain 0/1 0/1                                                                 testis 1/1 1/1                                                                placenta 0/1 1/1                                                            ______________________________________                                    

Note that, in this table and the table which follows "LUR 171" is SEQ IDNO:17, "1338" is SEQ ID NO:12, "1339" is SEQ ID NO:11, "LUR 162" is SEQID NO:18, "LUR 84" is SEQ ID NO:5 and "LUR 85" is SEQ ID NO:6.

EXAMPLE 8

It is known that certain MAGE genes are inducible with5-aza-2'-deoxycytidine, in both melanoma cells, and in different celltypes which do not normally express the genes See Weber, et al, CancerRes 54:1766-1771 (1994); DeSmet, et al, Proc. Natl, Acad Sci. USA93:7149-7153 (1996); DePlaen, et a, Genomics 40: (1997). Additionalagents may also be used to induce MAGE genes.

In order to determine if the MAG-1 genes parallel other genes in termsof inducibility, different types of cells were incubated for 72 hours inculture medium containing 1 μm 5-aza-2'-deoxycytidine ("DAC" hereafter),in accordance with DeSmet, et al, supra. The table which follows setsforth the result.

    ______________________________________                                               MAGE-B1           MAGE-B2                                                     LUR171-1338                                                                             1339-1338   LUR162-LUR85                                       (exon3-exon4) (exon1-exon4) (exon1-exon2)                                          -     +DAC    -       +DAC  -     +DAC                                 ______________________________________                                        Cell lines:                                                                     MZ2-MEL -  + - - - +                                                          SK23-MEL - - - - - -                                                          M1665/2-MEL - + - - - +                                                       LE92.11-RCC - - - - - +                                                       JAR - + - - - +                                                               LB23-SAR - + - - - +                                                          B-EBV - + - - - +                                                             Normal tissues:                                                               PBL-PHA - + - - - +                                                           Fibroblasts - - - - - +                                                       Dentriltic cells - + - - - +                                                ______________________________________                                    

"Nucleic acid molecule" as used herein refers to all species of DNA andRNA which possess the properties discussed supra. Genomic ("gDNA") andcomplementary DNA, or "cDNA" both code for particular proteins, and asthe examples directed to isolation of MAGE coding sequences show, thisdisclosure teaches the artisan how to secure both of these.

The four MAGE-B genes are spread over 40,352 kb in the 160 kb X-linkedcritical region defined for the DSS (Dosage Sensitive Sex reversal)locus involved in sex determination (Bardoni et al. Nature Genetics7:497-501 (1994)). This region is duplicated in patients with amale-to-female sex reversal phenotype. Genes in this region may beinvolved in X-linked disorders such as adrenal hypoplasis congenita andhypogonadism.

All isolated nucleic acid molecules which encode MAGE-B proteins, withthe exception of MAGE-B1, are encompassed by this invention. Thisincludes those nucleic acid molecules which hybridize to any of MAGE-B2,MAGE-B3, or MAGE-B4 under stringent conditions. As used herein, thisrefers to conditions such as hybridization with 5×10⁶ cpm/ml for 18hours at 65° C., followed by 4, 20 minute washes at 65° C., with eachwash using 2×SSC, 0.5% SDS and 1×Denhardt's solution, followed by twowashes at 0.2×SSC, 1% SDS (20 minutes, each wash), and , finally , twowashes at 68° C., 1% SDS, a varying concentration of SSC, each of thesewashes being for 20 minutes. The final concentration of SSC should be nogreater that 0.5×SSC, more preferably it is 0.2×SSC, and most preferablyit is 0.1×SSC.

Similarly, RNA molecules, such as mRNA can be secured, Again, withreference to the skilled artisan, once one has a coding sequence inhand, mRNA can be isolated or synthesized.

Complementary sequences which do not code for TRAPs, such as "antisenseDNA" or mRNA are useful, e.g., in probing for the coding sequence aswell as in methodologies for blocking its expression.

It will also be clear that one may manufacture biologically purecultures of prokaryotic and eukaryotic cell lines which have beentransformed or transfected with nucleic acid sequences which code for orexpress the MAGE-B molecules. Such cultures can be used as a source fortumor rejection antigens, e.g., or as therapeutics. This aspect of theinvention is discussed infra.

Cells transfected with MAGE-B coding sequences may also be transfectedwith other coding sequences. Examples of other coding sequences includecytokine genes, such as interleukins (e.g., IL-2 or IL-4), or majorhistocompatibilty complex (MHC) or human leukocyte antigen (HLA)molecules. Cytokine gene transfection is of value because expression ofthese is expected to enhance the therapeutic efficacy of thebiologically pure culture of the cells in vivo. The art is well aware oftherapies where interleukin transfectants have been administered tosubjects for treating cancerous condition. In a particularly preferredembodiment, cells are transfected with sequence coding for each of (i)MAGE-Xp molecule, (ii) an HLA/MHC molecule, and (iii) a cytokine.

Transfection with an MHC/HLA coding sequence is desirable becausecertain of the TRAs derived from MAGE-B may be preferentially orespecially presented only by particular MHC/HLA molecules. Thus, where arecipient cell already expresses the MHC/HLA molecule associated withpresentation of a TRA, additional transfection may not be necessaryalthough further transformation could be used to cause overexpression ofthe antigen, On the other hand, it may be desirable to transfect with asecond sequence when the recipient cell does not normally express therelevant MHC/HLA molecule. It is to be understood, of course, thattransfection with one additional sequence doe not preculdue furthertransfectin with other sequences.

The term "biologically pure" as used in connection with the cell linedescribed herein simple means that these are essentially free of othercells, Strictly speaking, a "cell line" by definition is "biologicallypure", but the recitation will establish this fully.

Transfection of cells requires that an appropriate vector by used. Thus,the invention encompasses expression vectors where a coding sequence forthe MAGE-Xp TRAP of interest is operably linked to a promoter. Thepromoter may be a strong promoter, such as those well known to the art,or a differential promoter, i.e., one which is operative only inspecific cell types. The expression vectors may also contain all or apart of a viral or bacterial genome, such as vaccinia virus or BCG. Suchvectors are especially useful in preparing vaccines.

The expression vectors may incorporate several coding sequences, as longas the MAGE-B sequence is contained therein. The cytokine and/or HLAgenes discussed supra may be included in a single vector with the TRAPsequence. Where this is not desired, then an expression system may beprovided, where two or more separate vectors are used where each codingsequence is operably linked to a promoter. Again, the promoter may be astrong or differential promoter. Co-transfection is a well knowntechnique, and the artisan in this field is expected to have thistechnology available for utilization, The vectors may be constructed sothat they code for the TRA molecule directly, rather than the MAGE-XpTRAP. This eliminates the need for post-translational processing.

As the foregoing discussion makes clear, the sequences code for "tumorrejection antigen precursors" ("TRAPs") which, in turn, are processedinto tumor rejection antigens ("TRAs"), Perhaps their most noteworthyaspect is as vaccines for treating various cancerous conditions. Theevidence points to presentation of TRAs on tumor cells, followed by thedevelopment fo an immune response and deletion of the cells. Theevidence in the art shows that when various TRAs are administered tocells, a CTL response is mounted and presenting cells are deleted. Thisis behavior characteristic of vaccines, and hence TRAPs, which areprocessed into TRAs, and the TRAs themselves may be used, either aloneor in pharmaceutically appropriate compositions, as vaccines, Similarly,presenting cells may be used in the same manner, either alone or ascombined with ingredients or yield pharmaceutical compositions.Additional materials which may be used as vaccines include isolatedcells which present the TRA molecule on their surface, as well as TRAPfragments, mutated viruses, especially etiolated forms, and transformedbacteria. "Fragments" as used herein refers to peptides which aresmaller than the TRA, but which possess the properties required of avaccine, as discussed supra. Another vaccine comprises or consists ofcomplexes of TRA and HLA molecule. Vaccines of the type described hereinmay be used preventively, i.e., via administration to a subject in anamount sufficient to prevent onset of a cancerous condition.

The generation of an immune response, be it T-cell or B-cell related, ischaracteristic of the effect of the presented tumor rejection antigen,With respect to the B-cell response, this involves, inter alia, thegeneration of antibodies to the TRA, i.e., which specifically bindthereto. In addition, the TRAP molecules are of sufficient size torender them immunogenic, and antibodies which specifically bind theretoare a part of this invention. These antibodies may be polyclonal ormonoclonal, the latter being prepared by any of the well recognizedmethodologies for their preparation which need not be repeated here, Forexample, mAbs may be prepared using an animal model, e.g., a Balb/Cmouse or in a test tube, using, e.g., EBV transformants. In addition,antiserum may be isolated from a subject afflicted with a cancerouscondition where certain cells present a TRA. Such antibodies may also begenerated to epitope defined by the interaction of TRA and HLA/MHCmolecules.

Review of the foregoing disclosure will show that there are a number offacets to the system which may be referred to as "tumor rejectionantigen presentation and recognition". Recognition of these phenomenahas diagnostic consequences, For example, the existence of specific CTLclones, or antibodies to the TRA makes it possible to diagnose ormonitor cancerous conditions (explained infra), by monitoring the CTLsin a sample from a subject, binding of antibodies to TRAs, or theactivity of anti-TRA CTLs in connection with subject samples, Similarly,the expression of nucleic acid molecules for TRAPs can be monitored viaamplification (e.g., "polymerase chain reaction"), anti-sensehybridization, probe technologies, and so forth. Various subjectsamples, including body fluids (blood, serum, and other exudates, e.g.),tissues and tumors may be so assayed.

A particular manner of diagnosis is to use an adaptation of the standard"tuberculin test" currently used for diagnosis of tuberculosis. Thisstandard skin test administers a stable form of "purified proteinderivative" or "PPD" as a diagnostic aid. In a parallel fashion, TRAs inaccordance with this invention may be used in such a skin test as adiagnostic aid or monitoring method.

The term "cancerous condition" is used herein to embrace allphysiological events that commence with the initiation of the cancer andresult in final clinical manifestation. Tumors do not spring up "abinitio" as visible tumors; rather there are various events associatedwith the transformation of a normal cell to malignancy, followed bydevelopment of a growth of biomass, such as a tumor, metastasis, etc. Inaddition, remission may be conceived of as part of "a cancerouscondition" as tumors seldom spontaneously disappear. The diagnosticaspects of this invention include all events involved in carcinogenesis,from the first transformation to malignancy of a single cell, throughtumor development and metastasis, as well as remission. All are embracedherein.

Where "subject" is used, the term embraces any species which can beafflicted with a cancerous condition. This includes humans andnon-humans, such as domesticated animals, breeding stock and so forth.

There are therapeutic aspects of this invention as well. The efficacy ofadministration of effective amounts of TRAPs and TRAs as vaccines havealready been discussed supra. Similarly, one may develop the specificCTLs in vitro and then administer these to the subject. Antibodies maybe administered, either polyclonal or monoclonal, which specificallybind to cells presenting the TRA of interest. These antibodies may becoupled to specific anti tumor agents, including, but not being limitedto, methotrexate radio-iodinated compounds, toxins such as ricin, othercytostactic or cytolytic drugs, and so forth, Thus, "targeted" antibodytherapy is included herein, as is the application of deletion of thecancerous cells by the use of CTLs.

The terms and expression which have been employed are used as terms ofdescription and not of limitation, and there is no intention in the useof such terms and expression of excluding any equivalents of thefeatures shown and described or portions thereof, it being recognizedthat various modifications are possible within the scope of theinvention.

    __________________________________________________________________________    #             SEQUENCE LISTING                                                   - -  - - (1) GENERAL INFORMATION:                                             - -    (iii) NUMBER OF SEQUENCES:  18                                         - -  - - (2) INFORMATION FOR SEQ ID NO: 1:                                    - -      (i) SEQUENCE CHARACTERISTICS:                                                 (A) LENGTH:   1866 - #base pairs                                              (B) TYPE:  nucleic a - #cid                                                   (C) STRANDEDNESS:  sing - #le                                                 (D) TOPOLOGY:  linear                                                - -  - -     (xi) SEQUENCE DESCRIPTION:  SEQ ID NO: - # 1:                    - - GAGTGTTGCA ACTGGGCCTG GCATGTTTCA GCGTGGTGTC CAGCAGTGTC TC -            #CCACTCCT     60                                                                 - - TGTGAAGTCT GAGGTTGCAA AAGGACTGTG ATCATATGAA GATCATCCAG GA -            #GTACAACT    120                                                                 - - CGAAATTCTC AGAAAACAGG ACCTTGATGT GAGAGGAGCA GGTTCAGGTA AA -            #CAAAGGGC    180                                                                 - - GAGGACCCGA GCGAGCTTAA GGCCAGTGGG GTGCAGCGTC TGGTCAGCCG AG -            #GGTGAATT    240                                                                 - - CTCAGGACTG GTCGGGAGTC AAGGTGCCAC ATCTCCTGCC TTTCTGCTCA CT -            #TTCCTGCC    300                                                                 - - TGTTTTGCCT GACCACAGCC ATCATGCCTC GGGGTCAGAA GAGTAAGCTC CG -            #TGCTCGTG    360                                                                 - - AGAAACGCCG CAAGGCGCGA GAGGAGACCC AGGGTCTCAA GGTTCGTCAC GC -            #CACTGCAG    420                                                                 - - CAGAGAAAGA GGAGTGCCCC TCCTCCTCTC CTGTTTTAGG GGATACTCCC AC -            #AAGCTCCC    480                                                                 - - CTGCTGCTGG CATTCCCCAG AAGCCTCAGG GAGCTCCACC CACCACCACT GC -            #TGCTGCAG    540                                                                 - - CTGTGTCATG TACCGAATCT GACGAAGGTG CCAAATGCCA AGGTGAGGAA AA -            #TGCAAGTT    600                                                                 - - TCTCCCAGGC CACAACATCC ACTGAGAGCT CAGTCAAAGA TCCTGTAGCC TG -            #GGAGGCAG    660                                                                 - - GAATGCTGAT GCACTTCATT CTACGTAAGT ATAAAATGAG AGAGCCCATT AT -            #GAAGGCAG    720                                                                 - - ATATGCTGAA GGTTGTTGAT GAAAAGTACA AGGATCACTT CACTGAGATC CT -            #CAATGGAG    780                                                                 - - CCTCTCGCCG CTTGGAGCTC GTCTTTGGCC TTGATTTGAA GGAAGACAAC CC -            #TAGTAGCC    840                                                                 - - ACACCTACAC CCTCGTCAGT AAGCTAAACC TCACCAATGA TGGAAACCTG AG -            #CAATGATT    900                                                                 - - GGGACTTTCC CAGGAATGGG CTTCTGATGC CTCTCCTGGG TGTGATCTTC TT -            #AAAGGGCA    960                                                                 - - ACTCTGCCAC CGAGGAAGAG ATCTGGAAAT TCATGAATGT GTTGGGAGCC TA -            #TGATGGAG   1020                                                                 - - AGGAGCACTT AATCTATGGG GAACCCCGTA AGTTCATCAC CCAAGATCTG GT -            #GCAGGAAA   1080                                                                 - - AATATCTGAA GTACGAGCAG GTGCCCAACA GTGATCCCCC ACGCTATCAA TT -            #CCTATGGG   1140                                                                 - - GTCCGAGAGC CTATGCTGAA ACCACCAAGA TGAAAGTCCT CGAGTTTTTG GC -            #CAAGATGA   1200                                                                 - - ATGGTGCCAC TCCCCGTGAC TTCCCATCCC ATTATGAAGA GGCTTTGAGA GA -            #TGAGGAAG   1260                                                                 - - AGAGAGCCCA AGTCCGATCC AGTGTTAGAG CCAGGCGTCG CACTACTGCC AC -            #GACTTTTA   1320                                                                 - - GAGCGCGTTC TAGAGCCCCA TTCAGCAGGT CCTCCCACCC CATGTGAGAA CT -            #CAGGCAGA   1380                                                                 - - TTGTTCACTT TGTTTTTGTG GCAAGATGCC AACCTTTTGA AGTAGTGAGC AG -            #CCAAGATA   1440                                                                 - - TGGCTAGAGA GATCATCATA TATATCTCCT TTGTGTTCCT GTTAAACATT AG -            #TATCTTTC   1500                                                                 - - AAGTGTTTTT CTTTTAATAG AATGTTTATT TAGAGTTGGG ATCTATGTCT AT -            #GAGCGACA   1560                                                                 - - TGGATCACAC ATTTATTGGT GCTGCCAGCT TTAAGCATAA GAGTTTTGAT AT -            #TCTATATT   1620                                                                 - - TTTCAAATCC TTGAATCTTT TTTGGGTTGA AGAAGAAGAA AGCATAGCTT TA -            #GAATAGAG   1680                                                                 - - ATTTTCTCAG AAATGTGTGA AGAACCTCAC ACAACATAAT TGGAGTCTTA AA -            #ATAGAGGA   1740                                                                 - - AGAGTAAGCA AAGCATGTCA AGTTTTTGTT TTCTGCATTC AGTTTTGTTT TT -            #GTAAAATC   1800                                                                 - - CAAAGATACA TACCTGGTTG TTTTTAGCCT TTTCAAGAAT GCAGATAAAA TA -            #AATAGTAA   1860                                                                 - - TAAATT                 - #                  - #                  -     #         1866                                                                  - -  - - (2) INFORMATION FOR SEQ ID NO: 2:                                    - -      (i) SEQUENCE CHARACTERISTICS:                                                 (A) LENGTH:   461 - #base pairs                                               (B) TYPE:  nucleic a - #cid                                                   (C) STRANDEDNESS:  sing - #le                                                 (D) TOPOLOGY:  linear                                                - -     (xi) SEQUENCE DESCRIPTION:  SEQ ID NO: - # 2:                         - - GCTGCGGCTG CGGGTGTTTC ATCCACAAAA TCTAAAAAAG GTGCCAAGAG CC -             #ACCAAGGT     60                                                                 - - GAGAAAAATG CAAGTTCCTC CCAGGCCTCA ACATCCACTA AGAGCCCAAG CG -            #AAGATCCT    120                                                                 - - CTAACCAGGA AGTCAGGGTC GTTGGTGCAG TTCCTGTTGT ACAAGTATAA AA -            #TAAAAAAG    180                                                                 - - TCCGTTACAA AGGGAGAAAT GCTGAAAATT GTTGGCAAAA GGTTCAGGGA GC -            #ACTTCCCT    240                                                                 - - GAGATCCTCA AGAAAGCCTC TGAGGGCCTC AGTGTTGTCT TTGGCCTTGA GC -            #TGAATAAA    300                                                                 - - GTCAACCCCA ACGGCCACAC TTACACCTTC ATCGACAAGG TAGACCTCAC TG -            #ATGAGGAA    360                                                                 - - TCCCTGCTCA GTTCCTGGGA CTTTCCCAGG AGAAAGCTTC TGATGCCTCT CC -            #TGGGTGTG    420                                                                 - - ATCTTCTTAA ATGGCAACTC AGCTACTGAG GAAGAGATCT G    - #                      - #  461                                                                     - -  - - (2) INFORMATION FOR SEQ ID NO: 3:                                    - -      (i) SEQUENCE CHARACTERISTICS:                                                 (A) LENGTH:   476 - #base pairs                                               (B) TYPE:  nucleic a - #cid                                                   (C) STRANDEDNESS:  sing - #le                                                 (D) TOPOLOGY:  linear                                                - -     (xi) SEQUENCE DESCRIPTION:  SEQ ID NO: - # 3:                         - - ATCACTGCAA CTAACAAGAA AAAAGTATCC TTTTCATCCC CTCTTATTTT GG -             #GGGCTACT     60                                                                 - - ATCCAGAAAA AGTCTGCTGG TAGGTCACGT AGTGCTCTCA AGAAGCCTCA GA -            #GAGCACTA    120                                                                 - - TCCACCACTA CATCTGTAGA TGTTTCTTAC AAAAAGTCAT ACAAGGGAGC CA -            #ACAGCAAA    180                                                                 - - ATTGAGAAAA AGCAAAGCTT CTCTCAGGGT CTATCCTCCA CTGTGCAGTC TC -            #ACACAGAC    240                                                                 - - CCTCTAACCA TGAAGACAAA TATGTTGGTG CAGTTCCTGA TGGAAATGTA CA -            #AGATGAAA    300                                                                 - - AAGCCCATTA TGAAAGCAGA TATGCTAAAA ATTGTCCAAA AAAGCCATAA GA -            #ATTGCTTC    360                                                                 - - CCTGAGATCC TTAAAAAAGC TTCTTTCAAC ATGGAGGTGG TGTTTGGTGT TG -            #ATTTAAAG    420                                                                 - - AAAGTTGATT CTACCAAGGA CTCCTATGTC CTTGTCAGCA AAATGGATCT CC - #CCAA            476                                                                       - -  - - (2) INFORMATION FOR SEQ ID NO: 4:                                    - -      (i) SEQUENCE CHARACTERISTICS:                                                 (A) LENGTH:   687 - #base pairs                                               (B) TYPE:  nucleic a - #cid                                                   (C) STRANDEDNESS:  sing - #le                                                 (D) TOPOLOGY:  linear                                                - -     (xi) SEQUENCE DESCRIPTION:  SEQ ID NO: - # 4:                         - - CATCACCTGC CCTTCTGCCT ACACTCCTGC CTGCTGTGCC TAACCACAGC CA -             #TCATGCCT     60                                                                 - - CGGGGTCAGA AGAGTAAGCT CCGTGCCCGT GAGAAACGCC AGCGGACCCG TG -            #GTCAGACC    120                                                                 - - CAGGATCTCA AGGTTGGTCA GCCTACTGCA GCAGAGAAAG AAGAGTCTCC TT -            #CCTCTTCC    180                                                                 - - TCATCTGTTT TGAGGGATAC TGCCTCCAGC TCCCTTGCTT TTGGCATTCC CC -            #AGGAGCCT    240                                                                 - - CAGAGAGAGC CACCCACCAC CTCTGCTGCT GCAGCTATGT CATGCACTGG AT -            #CTGATAAA    300                                                                 - - GGCGACGAGA GCCAAGATGA GGAAAATGCA AGTTCCTCCC AGGCCTCAAC AT -            #CCACTGAG    360                                                                 - - AGATCACTCA AAGATTCTCT AACCAGGAAG ACGAAGATGT TAGTGCAGTT CC -            #TGCTGTAC    420                                                                 - - AAGTATAAAA TGAAAGAGCC CACTACAAAG GCAGAAATGC TGAAGATCAT CA -            #GCAAAAAG    480                                                                 - - TACAAGGAGC ACTTCCCTGA GATCTTCAGG AAAGTCTCTC AGCGCACGGA GC -            #TGGTCTTT    540                                                                 - - GGCCTTGCCT TGAAGGAGGT CAACCCCACC ACTCACTCCT ACATCCTCGT CA -            #GCATGCTA    600                                                                 - - GGCCCCAACG ATGGAAACCA GAGCAGTGCC TGGACCCTTC CAAGGAATGG GC -            #TTCTGATG    660                                                                 - - CCTCTACTGA GTGTGATCTT CTTAAAT          - #                  - #                687                                                                     - -  - - (2) INFORMATION FOR SEQ ID NO: 5:                                    - -      (i) SEQUENCE CHARACTERISTICS:                                                 (A) LENGTH:   22 - #base pairs                                                (B) TYPE:  nucleic a - #cid                                                   (C) STRANDEDNESS:  sing - #le                                                 (D) TOPOLOGY:  linear                                                - -     (xi) SEQUENCE DESCRIPTION:  SEQ ID NO: - # 5:                         - - TAAAAAAGGT GCCAAGAGCC AC           - #                  - #                     22                                                                      - -  - - (2) INFORMATION FOR SEQ ID NO: 6:                                    - -      (i) SEQUENCE CHARACTERISTICS:                                                 (A) LENGTH:   20 - #base pairs                                                (B) TYPE:  nucleic a - #cid                                                   (C) STRANDEDNESS:  sing - #le                                                 (D) TOPOLOGY:  linear                                                - -     (xi) SEQUENCE DESCRIPTION:  SEQ ID NO: - # 6:                         - - TGAGGCCCTC AGAGGCTTTC            - #                  - #                      - # 20                                                                   - -  - - (2) INFORMATION FOR SEQ ID NO: 7:                                    - -      (i) SEQUENCE CHARACTERISTICS:                                                 (A) LENGTH:   21 - #base pairs                                                (B) TYPE:  nucleic a - #cid                                                   (C) STRANDEDNESS:  sing - #le                                                 (D) TOPOLOGY:  linear                                                - -     (xi) SEQUENCE DESCRIPTION:  SEQ ID NO: - # 7:                         - - AGTCTGCTGG TAGGTCACGT A           - #                  - #                      - #21                                                                   - -  - - (2) INFORMATION FOR SEQ ID NO: 8:                                    - -      (i) SEQUENCE CHARACTERISTICS:                                                 (A) LENGTH:   23 - #base pairs                                                (B) TYPE:  nucleic a - #cid                                                   (C) STRANDEDNESS:  sing - #le                                                 (D) TOPOLOGY:  linear                                                - -     (xi) SEQUENCE DESCRIPTION:  SEQ ID NO: - # 8:                         - - TCAGGAACTG CACCAACATA TTT           - #                  - #                    23                                                                      - -  - - (2) INFORMATION FOR SEQ ID NO: 9:                                    - -      (i) SEQUENCE CHARACTERISTICS:                                                 (A) LENGTH:   20 - #base pairs                                                (B) TYPE:  nucleic a - #cid                                                   (C) STRANDEDNESS:  sing - #le                                                 (D) TOPOLOGY:  linear                                                - -     (xi) SEQUENCE DESCRIPTION:  SEQ ID NO: - # 9:                         - - AGGGATACTG CCTCCAGCTC            - #                  - #                      - # 20                                                                   - -  - - (2) INFORMATION FOR SEQ ID NO: 10:                                   - -      (i) SEQUENCE CHARACTERISTICS:                                                 (A) LENGTH:   22 - #base pairs                                                (B) TYPE:  nucleic a - #cid                                                   (C) STRANDEDNESS:  sing - #le                                                 (D) TOPOLOGY:  linear                                                - -     (xi) SEQUENCE DESCRIPTION:  SEQ ID NO: - # 10:                        - - CAGGAACTGC ACTAACATCT TC           - #                  - #                     22                                                                      - -  - - (2) INFORMATION FOR SEQ ID NO: 11:                                   - -      (i) SEQUENCE CHARACTERISTICS:                                                 (A) LENGTH:   20 - #base pairs                                                (B) TYPE:  nucleic a - #cid                                                   (C) STRANDEDNESS:  sing - #le                                                 (D) TOPOLOGY:  linear                                                - -     (xi) SEQUENCE DESCRIPTION:  SEQ ID NO: - # 11:                        - - GTGGTGTCCA GCAGTGTCTC            - #                  - #                      - # 20                                                                   - -  - - (2) INFORMATION FOR SEQ ID NO: 12:                                   - -      (i) SEQUENCE CHARACTERISTICS:                                                 (A) LENGTH:   23 - #base pairs                                                (B) TYPE:  nucleic a - #cid                                                   (C) STRANDEDNESS:  sing - #le                                                 (D) TOPOLOGY:  linear                                                - -     (xi) SEQUENCE DESCRIPTION:  SEQ ID NO: - # 12:                        - - GTCAGATTCG GTACATGACA CAG           - #                  - #                    23                                                                      - -  - - (2) INFORMATION FOR SEQ ID NO: 13:                                   - -      (i) SEQUENCE CHARACTERISTICS:                                                 (A) LENGTH:   21 - #base pairs                                                (B) TYPE:  nucleic a - #cid                                                   (C) STRANDEDNESS:  sing - #le                                                 (D) TOPOLOGY:  linear                                                - -     (xi) SEQUENCE DESCRIPTION:  SEQ ID NO: - # 13:                        - - AATGTGTTGG GAGCCTATGA T           - #                  - #                      - #21                                                                   - -  - - (2) INFORMATION FOR SEQ ID NO: 14:                                   - -      (i) SEQUENCE CHARACTERISTICS:                                                 (A) LENGTH:   24 - #base pairs                                                (B) TYPE:  nucleic a - #cid                                                   (C) STRANDEDNESS:  sing - #le                                                 (D) TOPOLOGY:  linear                                                - -     (xi) SEQUENCE DESCRIPTION:  SEQ ID NO: - # 14:                        - - ATTATGTTGT GTGAGGTTCT TTCA          - #                  - #                    24                                                                      - -  - - (2) INFORMATION FOR SEQ ID NO: 15:                                   - -      (i) SEQUENCE CHARACTERISTICS:                                                 (A) LENGTH:   40352 - #base pairs                                             (B) TYPE:  nucleic a - #cid                                                   (C) STRANDEDNESS:  sing - #le                                                 (D) TOPOLOGY:  linear                                                - -     (xi) SEQUENCE DESCRIPTION:  SEQ ID NO: - # 15:                        - - AAGCTTCAGA GTTGAGAGAC TAAGCCTATA ATGGAAAAGT GCTCTTAACT  - #                  50                                                                         - - TTGGAGAAAA CTGGAAAGAA GCTCCACCAG GGGCAGCACT GGAAAGTGAC  - #                 100                                                                         - - TATGCTCTTG TCCCAGTGCA GATGAACACA GTGCAGAAAC TAGCTGCTTT  - #                 150                                                                         - - ATATACATTG TATCCAGTGA GCTTCGGAGA AATAAGGATG ATACTCCCTT  - #                 200                                                                         - - AAGAAGAAAT GATAAGAAGC CGCTGGGTAC TCTGCACTGG GCTGGAAGAG  - #                 250                                                                         - - TTAAGAGTCA ATTTCATTAA AAGGAGCATT CAAATTAGGC TATCATGTAC  - #                 300                                                                         - - ATAATTTGGC AAGCTCTAGG CAAGTTCTAG GTGTTTGGTG CTTGTTAAAT  - #                 350                                                                         - - TAATGAAAAT AAAGGTGATT TAGATGGAAG TGACACTGGG AGGGAAGGAA  - #                 400                                                                         - - GTTGTTAGTC CTTGACGCAA ATGCGTTGTG TTGCACCTAA ACTGGAGAAG  - #                 450                                                                         - - TCTACCTCAC ATTGAATAAT TTATCATTTA ATGGGGAAAT ATTACTTGAT  - #                 500                                                                         - - TTTTCGTGCT AATCTGCATT TTGGCTTATT GGGTTTTCTT TCTTCTAGAA  - #                 550                                                                         - - TGCTGCATAT TCTCTGACAT CTGTTGGATT AAGAAAAAGC AATCACAGTG  - #                 600                                                                         - - GGATGGGTGG TATCATCAAG TTAATCATAG GTAATAGCTG CCATGTTGAA  - #                 650                                                                         - - ACCTCAATAT GGGCCAAGTA TTGTGCCAGG TGCTTTACAT ACATTAACCA  - #                 700                                                                         - - CATTTCACCA ATTCCACAAG GAAGGGGCTT ATCAAACCTA TTTCACAGAT  - #                 750                                                                         - - GCAGAGCCTG AGGCTCACAG AGTTTAGTAA TGTGCCTGAG TTCACATAGC  - #                 800                                                                         - - TGGTAAGTGG CAGGGCTGAA ACCAGACCCC CAGTCTGGAT TAATCTACAT  - #                 850                                                                         - - CCCACACTGT TCCAGTCTTC CCATTCTGAG GCTAACCTCA TGTTGCTTCA  - #                 900                                                                         - - TTCATTTTTT CTCTCTATTT TTGAAAAATG TATCTCAGGC AATAAAAAGA  - #                 950                                                                         - - AGGACTCTGT GCCCATACTA CTGGGTCAAA TATATTCCCA GACCAATATG  - #                1000                                                                         - - AATGCCAAAA TAAATGAGAG GCATAAATAA AAAAAAGAAA AATAGTACCT  - #                1050                                                                         - - GGTGTCAATA CGTTCATCTA CACAAGCAAT ATCAGGTAAT CTCAAAAGAT  - #                1100                                                                         - - CAGAAGCTCC CAAAATCATC CTGGACAGTC TCTTCCCTGT AGAAAATAAA  - #                1150                                                                         - - TGTGTCAGAA CAGAATTTTC GTGAGAATCT AAGTCTAGCA AGATGGAAAA  - #                1200                                                                         - - AATCAGCATG TTTTTTTCTC TGACAGCCCA CTACCTGTCC TGGAGCTTCT  - #                1250                                                                         - - GCCTTGTGCT TCAGCTTCCC TATCTCACAT CACTCCCGGA ACACGGCTCC  - #                1300                                                                         - - TTCTCTGCAA GGTTTGCAGT AGAAAAACTG GTCATGTTTG CAGGGATGTG  - #                1350                                                                         - - ACATTTCCCA GAAAGCCTTT AATGAAAGAT GATCCCCAGT TCAGAGGACA  - #                1400                                                                         - - TGGGAAACCA ATATCTCAGA ATATATGAGG TTATGCATAG ACTGACTCTC  - #                1450                                                                         - - ATCTGCTCTC AGACCTAGAG AGCTCGAGAC AGAACTGTCA GGCTGAGCAT  - #                1500                                                                         - - CCACTCATTT AATCTTCAGA CCTCTCATGG ACATGGCCTA AGAAGGTCAA  - #                1550                                                                         - - CTTCATGTCA GTAGATGCAG TTCAGCCTCT GATAGATATG AACGTGAGGA  - #                1600                                                                         - - CCCTGAATGA AGATGATGGA AGGACCCAGC CAGAACTGTG GGGTCCCAAA  - #                1650                                                                         - - AAGTCCAGCT CAGGCTGTCA GCCCTTCAAA GTCCAGAACA GCCCTGGCTA  - #                1700                                                                         - - AATGTAGCTC ACCACAACTT CCAGTTCAGA GGTCGGCAGA GGGTGAGTAC  - #                1750                                                                         - - CTTGATCTGA TGGGAACAGC CTCAGGTCAG TGGAGGATGG AGTGCCCCAA  - #                1800                                                                         - - GACTGGCCAG GTGTCATGGT GAGGACCCTG AAGAGAAATA AGAGAACTGT  - #                1850                                                                         - - TATAGGACCA ACGAGTTTCT GTGTCAGCTG CAAAGTAACA GACCCACTAT  - #                1900                                                                         - - AATAAAACAG CAAAGTTTGC AGCAAAGAGT TTAATGATTG CAGGGTGCCA  - #                1950                                                                         - - AGTGAGGAGA TGAGAAGAGA CTCTCAAATT CATCTCCCCA AGGAGTTCTG  - #                2000                                                                         - - GGCTGGGATT TGCAAGGAGA TCATGGAGGG CGAGGGGCTG GAGAACTGAG  - #                2050                                                                         - - GTTGTTGATT CGTTGGGGTA AAGGGGATGA AATCATCAGA ATGTGGAAAC  - #                2100                                                                         - - TGCGTTTTTT TTTTTTTAGT CAGCTCCTGT GGGGTCTTTT GGACCAGCTG  - #                2150                                                                         - - ATGTCAGTAG GGTCCTTTAG AGCAGCTGGC ATCAGTGGGG TCCTTCAGAC  - #                2200                                                                         - - CAGCTGAGTC ATTAGAATCA TCAGTATGCA GGACCTTAAG GAATATCTCA  - #                2250                                                                         - - AAGAAAAAAA GTTTCATTAT GTTAAAATTG TTATCTATAG AGCAATTAAG  - #                2300                                                                         - - GGCAACTATA ATCTAGTAAC AGGATCTACA TGATTCTGGG ATAATAGGCA  - #                2350                                                                         - - CCAAACTACT ATGAGGAAGC AGGTCAGAGA GCAGCTGACT TCATGATTAA  - #                2400                                                                         - - TGCTGAATGT TCTGCAAGCT TGGCGTATCT TCATTTATCT CCCTTCCCTC  - #                2450                                                                         - - TTCCCTGATT AATTTTATAC AGTTCATAGG GGCAGTTTCA AAACCACCCA  - #                2500                                                                         - - CCCAGAACAG AGGAGCCCAC AAATTCCACC AATCTGTCAC CCTTGGGGAG  - #                2550                                                                         - - CCCAGAAGAG AATTATGAGG TGGAGAAATC CCATCAATAT GCCTCGTGTG  - #                2600                                                                         - - TTTCACAGAG GTGAAGACCT TGGACTAGTG GAAATGGCCC TAGGTCAAAG  - #                2650                                                                         - - AGACAAAACC CACGCCCTAA CAGGAATCAA GGTGAGGATC CTAAGTGTTA  - #                2700                                                                         - - CGAGGGGGCT CTCCCCCTTG CAACACCAGC AGAGGGGGCC AAACAGAGCC  - #                2750                                                                         - - CTCGCCATTG TTAGCACCGA GAGCCCCAAA ATAAGTATCT CTGAATCCCT  - #                2800                                                                         - - TCATTCCACC TGTATAGTCT CGGGGAAGGA AGGGCCTTTG TCTGAAGAGG  - #                2850                                                                         - - GTGACCCAGT TGTGCAGAGA GAGAGTCTTG GCTTTCACGG GAATCAAGGT  - #                2900                                                                         - - GAGGACTCTG AGGGCGGATG AGAAGACCTC TCCCCAAAAA AGGCACATTC  - #                2950                                                                         - - ACAGAGCCCT GCCGCTGCTG TCAGGCCTGT GAGGCCAGGC AGGGGTGGCC  - #                3000                                                                         - - TGTTTGGCAC GCTTAGATTT CCACAGTGGG GGCTGAGGGA GGTGGGGGTA  - #                3050                                                                         - - TTGTTTGGAG GCTGGCGGAT TTGGGTCAGC ACGCATATTC GTCCCAGGCT  - #                3100                                                                         - - GCTAGATACT GAGGTGAGGA CCCTAGTGGA GACGAAGGGA CCAGCAACGC  - #                3150                                                                         - - TAGAACAGTG ACGTCCGGTA GCGTCCAGCC GTCAGCCCCT CAGACGCCAC  - #                3200                                                                         - - GGGCTGCCGG ATGTGAGTCA TCCTGACTTC CGCTTTGAAA AAAAAGACCC  - #                3250                                                                         - - GAGCGGATGT GGCTCATCCT GACTTCCGCT TTGGAGGCGA GGACCCGAGC  - #                3300                                                                         - - GAGTGTAGGG GGTGCGGCGT CTGGTCAGCC AGGGGTGAAT TCTCAGGACT  - #                3350                                                                         - - GGTCGGCAGT CAAGGTGAGG ACCCTGAGTG TAAACTGAAG AGACCACCCC  - #                3400                                                                         - - CACCTGTAAC AAAGAGGGCC CCACTAAGTC CCGCTTCTGC ATTTGGTCCT  - #                3450                                                                         - - GAGAGGCTCC GGTAAAGCCG TCCGGCAATG TTCCACCTGG AAAGTTCCAG  - #                3500                                                                         - - GGCAGGGGAA GGGTGGGGGG AGGGGCAGTC GCGGGGGAAG GAGGTTTGGA  - #                3550                                                                         - - CGCAGGGAAT AGGCCTCATT CTGCACGTAG GGTGGGTGTA GGCCCTAACT  - #                3600                                                                         - - GAAATCAATT TGAGGGCCCT AAATGTGGAC TGAAGAGAAC ATCTCCTACC  - #                3650                                                                         - - CTTAACAAAG GTGGCCTCAC TAAGTCTCGT CCCTGAAGAC GGCTCTGGGA  - #                3700                                                                         - - GGCCCCAGCA AAGCTGTGCC TGGAAAGTCT CAGGGAGGGG AGGGCCTTGA  - #                3750                                                                         - - ACCTAGGAAG CAGCCCTGCT TCTGCTCATA TGGCATCAAC CTAAAACCTA  - #                3800                                                                         - - ACTGGGGTTA AGGTGAGGAC TCTGAGTATT AATGAGCGGA CCTCTACCCA  - #                3850                                                                         - - AAAGAGGAGT CATGCTGGGC AGTTCCCAGG CCGAGGTGAC ACTGGGTTTT  - #                3900                                                                         - - AGATGGTCCC AGGCTGACAT GACAAATGCA GAGCAACCTG ACTTCCTCTC  - #                3950                                                                         - - CCGGGACTAA AGAAGTTGAG GGCTACATCC AAGACCCACA TCTGCTGCCA  - #                4000                                                                         - - GCAATAGGAA GGTCAGGGCA AAGCTAGCAA GCTGAGATGC TCTCTAGTTT  - #                4050                                                                         - - CCTCTGGAAG GTGGTCTCAG GGAGGTGAGG GTCTGGTGTA TGGGGTAGGT  - #                4100                                                                         - - CTAATGCGAC AGAGAGGCCC AGTCTCTAAC AGGAAGCAAG GATGTGACAC  - #                4150                                                                         - - TGAGTGATGA GGAGGGGACT CCAACCAGAA AGAAGTGCCA TATAGAGCCC  - #                4200                                                                         - - ACTCTTGTTG TCAGACCAGG GAGACCTGAG CATGGCATGT TGAGTTGTAC  - #                4250                                                                         - - TCACTCTCCC CAAGACATCT CAGAGAAGTG AACGACCCCA TTAAAGAGGG  - #                4300                                                                         - - CAGCCTGGCC GGGCGCGGTG GCTCACGCCT GTAATCCCAG CACTTTGGGA  - #                4350                                                                         - - GGCCGAGACA GGCGGATCAC GAGGTCAGGA GATCGAGACC ATCCTGGCTA  - #                4400                                                                         - - ACACGGTGAA ACCCTGTCTC TACTAAAAAT ACCAAAAAAA AAATTAGCGG  - #                4450                                                                         - - GGAGCGGTGG CGGGCGCCTG TAATCCCAGC TACTCGGGAG GCTGAGGCAG  - #                4500                                                                         - - GAGAATGGTG TGAACCTGGG AGGCGGAGTT TGCAGTGAGC CGAGATAGAG  - #                4550                                                                         - - CCACTGCAGT CCGGCCTAGG CGAAAGGGCG AGACTCCGTC TCTTAAAAAA  - #                4600                                                                         - - AAAAAAATAA AAAGGCAGCT TGAGGTCAGC AGAGGGAGGG TTTCCAGGTT  - #                4650                                                                         - - GTGCCAGATG TTATGATAAG AACTCTGACG ACTGCAGGGG GCTCCCACCC  - #                4700                                                                         - - CATATTAGTG GAGCCACACA TCCTCAGTAC TGTCAGCTCT GAGAGACCCC  - #                4750                                                                         - - AGGCAGAAAT GTGAAACAGA GTGCCCATCA GTTCCCACTC AAGGGTAACA  - #                4800                                                                         - - GGGAAATGAG AGTTTAATCT GAGGGTGTGA TCACTTGTCA ACAGAAGTAA  - #                4850                                                                         - - TGTACTAATT GGTCCATTCT GGCATTACTA TAAATACCCA AGACTGGGTA  - #                4900                                                                         - - ATTTACAAAG AAAAGAGGTT TAATTGGCTC ACAGTTCTGC AGGCTGTACA  - #                4950                                                                         - - GGAAGCATGA TGCTGGCATT TGCTCAGCTT CTGGGAGCAG GGCTCAGGAA  - #                5000                                                                         - - ATTTACAATC ATGGCAGAAG GGGAAGGGGA AGCAGACATG TTACATGGCC  - #                5050                                                                         - - AGAGCAGGAT CAAGAGGCCA GGGGAGGCGC TACACACTTT TAAATGACCA  - #                5100                                                                         - - GATCTCATGA GAAGTCATCG TTAAGGGGAA TGGTGCTAAA CCATTCATGA  - #                5150                                                                         - - AAAACCTGCC CTCATGATCC AGTCATCTCC CACCAGGCTC CACCTCCAAC  - #                5200                                                                         - - ATTGAGGATT ACAGTTAGAC ATGAGATTTG GTGAGGATAC AGATCCAAAC  - #                5250                                                                         - - CATATCAGGG AAGAATCTTA GACCCTCTCA TGAGTCAAAT TTAGAGGCTG  - #                5300                                                                         - - AGTTGGGACT GTATGTGGGA CCATCTATCA CAGGGCAGTT ATCTGCCTGC  - #                5350                                                                         - - CACAGCTTTT ACTTTTGGGA AGACCACATT TGGTCAGATA AGGCCACCCT  - #                5400                                                                         - - CACTTCCTCC TATGGGTTCT CAGGGATGTT CACTCTTACC ATGACGACAT  - #                5450                                                                         - - AGGCCTCAGT TCAATAAAAA GGAGAGCTCC TCTACCAGGA GTCAAATTGA  - #                5500                                                                         - - GGAATCTGAG AATTCAAGGA ACCACTGAAC CCTTAACAGT GGAGACATCA  - #                5550                                                                         - - AAGAATCCAG TCCAACCCTT TATTTTCAGC CCTAGAAAGC TCAGGAAAGG  - #                5600                                                                         - - GTTATCAGGT TTAGTGTCCC CCTCTCTTTT TTATATAAGA TCTAAGAGAG  - #                5650                                                                         - - GTGAGATTCT TAGTCTGACG GTGTAGCCAC CACTCAGCAG AAGGGGGCTG  - #                5700                                                                         - - GTGCCAAGCC CTACATGAAG CCAAGGTGGG GACCTTGAAT GAGAGCTGAA  - #                5750                                                                         - - GACTCTAATG ATTCCAAGAC ATCAAAGACC CCACTGAACT CTCAGCACTG  - #                5800                                                                         - - GGTGTCACTT CTCAGGGTGG TGGTTGAGGC ACCCCTTCAA TTTCTTCTCA  - #                5850                                                                         - - TGAGTCCCAG GGTGCTTTGA GGTGTCAAAT TCACAGTAGC GGAAGGGAGT  - #                5900                                                                         - - GGCTCAGGCC TGCAAGACTT CATATGATAA TCCTAAAAGT TAACTGATAA  - #                5950                                                                         - - AACCCCAAAC ACCAGAAGAC AGGAGGCCGC ACTGCCAGTA CCTCCTGTCA  - #                6000                                                                         - - CCATAGTAAG CCCAAGGAAG GGCTGACAGA ATGCAGTCTG AAACTCACTG  - #                6050                                                                         - - TAGGGGTTCT GTGGTTCTTC TCTGGTGTCT CAGGGGAAAG GATAACCAGG  - #                6100                                                                         - - ACGACAGGAG CCTTGTGGCT TTCTAGAACA GTGCCTTCAG GGAAACTTGC  - #                6150                                                                         - - AAAGGCAGGC TTCCTTCAGT AAAGCTAAGA TGGGGTCTTC CAGCTGAAGG  - #                6200                                                                         - - TGCTCACAGA TCTCATTCTC CCATCTCCAG GTATACTAAC CATCTATTCT  - #                6250                                                                         - - TCTGCCCACA TTTCTTGGTT TACCCAGCCA TCATGCCTCG TGGTCAGAAG  - #                6300                                                                         - - AGTAAGCTCC GTGCCCGTGA GAAACGCCGC AAGGCCCGAG ATGAGACCCG  - #                6350                                                                         - - GGGTCTCAAT GTTCCTCAGG TCACTGAAGC AGAGGAAGAA GAGGCCCCCT  - #                6400                                                                         - - GCTGTTCCTC TTCTGTTTCT GGGGGTGCTG CTTCAAGCTC TCCTGCTGCT  - #                6450                                                                         - - GGCATTCCCC AGAAGCCTCA GAGAGCCCCA ACCACTGCCG CTGCTGCGGC  - #                6500                                                                         - - TGCGGGTGTT TCATCCACAA AATCTAAAAA AGGTGCCAAG AGCCACCAAG  - #                6550                                                                         - - GTGAGAAAAA TGCAAGTTCC TCCCAGGCCT CAACATCCAC TAAGAGCCCA  - #                6600                                                                         - - AGCGAAGATC CTCTAACCAG GAAGTCAGGG TCGTTGGTGC AGTTCCTGTT  - #                6650                                                                         - - GTACAAGTAT AAAATAAAAA AGTCCGTTAC AAAGGGAGAA ATGCTGAAAA  - #                6700                                                                         - - TTGTTGGCAA AAGGTTCAGG GAGCACTTCC CTGAGATCCT CAAGAAAGCC  - #                6750                                                                         - - TCTGAGGGCC TCAGTGTTGT CTTTGGCCTT GAGCTGAATA AAGTCAACCC  - #                6800                                                                         - - CAACGGCCAC ACTTACACCT TCATCGACAA GGTAGACCTC ACTGATGAGG  - #                6850                                                                         - - AATCCCTGCT CAGTTCCTGG GACTTTCCCA GGAGAAAGCT TCTGATGCCT  - #                6900                                                                         - - CTCCTGGGTG TGATCTTCTT AAATGGCAAC TCAGCTACTG AGGAAGAGAT  - #                6950                                                                         - - CTGGGAATTC CTGAATATGT TGGGAGTCTA TGATGGAGAG GAGCACTCAG  - #                7000                                                                         - - TCTTTGGGGA ACCCTGGAAG CTCATCACCA AAGATCTGGT GCAGGAAAAA  - #                7050                                                                         - - TATCTGGAGT ACAAGCAGGT GCCCAGCAGT GATCCCCCAC GCTTTCAATT  - #                7100                                                                         - - CCTGTGGGGT CCGAGAGCCT ATGCTGAAAC CAGCAAGATG AAAGTCCTGG  - #                7150                                                                         - - AGTTTTTGGC CAAGGTAAAT GGTACCACGC CCCTGTGCCT TCCCAACCCA  - #                7200                                                                         - - TTACGAAGAA GCTTTGAAAG ATGAAGAGAA AGCCGGAGTC TGAGCCAGAG  - #                7250                                                                         - - TTGTAGCCAG GCCTTGCACT ACTGCCATAG CCAATCAATC TCCCAAAGCC  - #                7300                                                                         - - AAGTTTACCT GCTGTTCTCA CCCCCAATGA GGTCTTAGGC AGATTCTTTA  - #                7350                                                                         - - CTTTGTAATT CAAAAGGCCT GTTAACCTTT GTTCTTGTTA TGCATGAATA  - #                7400                                                                         - - ACTTGTTGAC TTTTTTTTTT TCTCTTTTTC AACTAGTGTT TCAACAGGTT  - #                7450                                                                         - - TATTTAGATT CAGAATGTAA ATTTACAAAT GATATAGATC ACCCTGTTAT  - #                7500                                                                         - - TGCTGTTTTT CAGGGACAGT AGAAAGTGTT TTGTTTTTTG AGTGAAACAA  - #                7550                                                                         - - CTTATTAATA AAAATCCTTA AATCACTTTT GTAATCCAGG ACAAGAAAAT  - #                7600                                                                         - - GTGGCATTAG AGTAGAAATA TCTTTGGAAA TGTGAAAGAC CCCATAGTGA  - #                7650                                                                         - - AATATTTGGG ATCAGAAGCC AGAGGTGTAA AAGTGGTCAA TTCTTGGTTT  - #                7700                                                                         - - ACTTCATTTA ATCTTTCTTT TCATAAAGAT ACATACCTGG ATTTGTTTAT  - #                7750                                                                         - - GTTATTCAAG AATGTGTGAG AAATTAAACC ATAGTTAGTT AATCCTCTTG  - #                7800                                                                         - - TTTATGGCTC TTACTTTAAA TATTTTAATT GAGCATCTGT TCTTTGGGAG  - #                7850                                                                         - - TCTTCCTGCT TGTACTGGGA ATGTTTAGTC AAAGAAGACC AAGCTTGTGC  - #                7900                                                                         - - TCATGCAATT GTAGATTCTA GGAGAAGCTG TCATCATGGA AGGTGAGACA  - #                7950                                                                         - - CTTCATAAAA AATAAAAGAT AGGAAAAAGA AGTGATGAAA GCAATAGTTT  - #                8000                                                                         - - TCATTGCTAA GCAGCATTTT GGCTGAATGG GGTTTTTCAG TCTTAGAATG  - #                8050                                                                         - - TTGCGTATTC TCAGCCGGGT GCAGTTGCTC ACATCTGTAA CTCCCAGCAC  - #                8100                                                                         - - TTTGGGAGGC TGAGGTGGGT GGATCACCTG AGGTCGGGAG TTCGAGACCA  - #                8150                                                                         - - GCCTGACCAA CATGGAGAAA CCCTGTCTCT ACTAAAAATA CAAAATTAGC  - #                8200                                                                         - - CAGGCGTGGT GGCGCATGGC TGTAATGCCA GCTACTCGGG AGGCTAAGGC  - #                8250                                                                         - - AGGAGAATCA CTTGAACCCA GGAGGCGGAG GTTGCTGTGA GCCGAGACTG  - #                8300                                                                         - - CGCCATTGCA CTCCAGCCTG GGCAACAAGA GCGAAACTCC CTCTAAAAAA  - #                8350                                                                         - - AAAAAAAAAA AAAAAAAAAA AAATTGTTGC ATATTCTCTG AGATATCCTG  - #                8400                                                                         - - GATTTTTTTT TTTTAATCTC AACTGGAATG AAGGTAATGT CTCTGATCAA  - #                8450                                                                         - - AATGATAGTG GTAATTGCCA CTTCCTGATG TCTCAGTTGA CGAGACAGGC  - #                8500                                                                         - - ACATTTGCAT GCATTACATA TATTCCACCA GTACTACGAG ATGGGGCTTA  - #                8550                                                                         - - TCAAACCCAC TTTACGGTAA AGAGTCTGAG GCTCACTGAG TTTGGTAATT  - #                8600                                                                         - - TGCCAAAGTT TACAGGGCCA GTAGTGAAAG GGTTGGGATA AGCCTCCTGA  - #                8650                                                                         - - TCTGAATTAC CCTAGAGCCT ACCCTGTTCC ACCCCTCTCA GCCTGAGGCA  - #                8700                                                                         - - AACCTTGAAA AAATATTTCC GTTTTCTTCT CATTACTGCG GAATGTGTTT  - #                8750                                                                         - - CAGGTGATAA AAAGAATAAC CTTGTAACCC AAACTAGTAG ATTGGAATAC  - #                8800                                                                         - - ATAGCCACAG CAACAATAAT ACCAAAAAAA CTGAGAGGTA TAAATAAGTA  - #                8850                                                                         - - AAAGAGGCAA TATTCAGTGA CAGTATGATT ATCTACATTC GCAATGTTAG  - #                8900                                                                         - - ATAACCTCAA AAGATCAGGG GCTCCCAAAT CATCCTGAAC AATCTCTTCC  - #                8950                                                                         - - CTGTAGAAAA TAAATCTATC AGAACAGAAA TTACATGAGA ATGTAAGGCT  - #                9000                                                                         - - GGCTGATAGA AGAAATAGAT CAATGTATCT TATCTCTGGC TGCCCATCTC  - #                9050                                                                         - - CAATCCTAGG CCCCCTGCTT TGTGCTACAG CTTTGCGGTA TCACACCACT  - #                9100                                                                         - - CCTAGGACAG AGACCCATTC TCTGCAATGT TTACAGAGAG GAAATTGTTC  - #                9150                                                                         - - TAGAATTTGC AGAGATGTGG CATTTTGCAG GAAGCATTTA ATCCAGAGAC  - #                9200                                                                         - - AGAGCCAGAT GAGGGCTGGG AGACCACATC CCAGAATATC AGGCAGGGGT  - #                9250                                                                         - - TTACATAGAC CATTCCCTTC TGCTCTCAGA CACGGTCCTA GGCTGAGCTG  - #                9300                                                                         - - TCAGTCTGAG CATCCCCTCA CATATTCTTC AGGTGTCTGA GAGACATAAG  - #                9350                                                                         - - AGCCCTAAGG GCAGCATCCT CAAGTCAGTA GGGAAAGGAA TCCCAGCCTC  - #                9400                                                                         - - TTATAGGAAT GAAGGTAATA AACCTAAATG AGGGTGATGG AAGGACCCAG  - #                9450                                                                         - - CCAGAACAGT TGGGGGCCTG TAAAGTCACG TTCCAATTGT AGTCTTGTGA  - #                9500                                                                         - - GTCCAGAACC GTCCTGGTGG ACACTGACTT CCAATTCAGG TCCCAGCAAG  - #                9550                                                                         - - GTGAGGACTT TGGTCTGAAG GCTATGGCCT CAGGTCAGTA GGGAGTGGAG  - #                9600                                                                         - - TCCCAGGAAG GGGGCAGAGT CAAGGTGAAG GCCTGGCATG TGGATATGGG  - #                9650                                                                         - - GACCACTCAA CCCATAACTG GCTGGCGTGC CTCTAGTCAT TCCTTTGCTA  - #                9700                                                                         - - TTAGCCCTAG GAGGCCTAGG GAGGAGCTGG CAGGCCCAGG TGCCCGTGTC  - #                9750                                                                         - - TTCTTCCTGG AGAATCTCAA GGAGATGAGA GACTTGATTT ACAGGCTGGC  - #                9800                                                                         - - CTCCCAGTTA AGCAAAGAGT GGAATCCCAG ACCATGATAG GTATTAAGGT  - #                9850                                                                         - - AAAAATCTCA TATGAGGAAA GAGGCAAACA ACTGCTAGAG AGGTGAACAG  - #                9900                                                                         - - AACAAGGACG TCCTTAGAAA GTTATGCCGG GCCAGACGCG GTGGCTCATG  - #                9950                                                                         - - CCTGTAATCC AGCACTTTGG CAGGCCGAGG TGGGCGGATC ATTTGAGGTC  - #               10000                                                                         - - AGGAGTTCAA GATCAGCCTG GCCAGCATGG CAAAAACCCG TCTCTACTTA  - #               10050                                                                         - - AAAAATACAA AAATTAGCTG GGCGTGATGG TGGGCGCCTG TAATCCCAGC  - #               10100                                                                         - - TACTTGGGAG GCTGAGGCAG GGAGAATTGC TTGAACCTGG GAGGCGAAGC  - #               10150                                                                         - - TTGCAGTGAG CTGAGATCAC GCCACTGCAC TCCAGCCTGG GCAACAGAGC  - #               10200                                                                         - - GAGACTTTAT CTTAAAAAAA AAAAAAGAAA AGAAAAGAAA GTTATGCCCT  - #               10250                                                                         - - GGGAGGCCAT AAGCATAGCT TTCAGGCTGA GGTTACCCCA CATTCGCTGA  - #               10300                                                                         - - GGGGAGGGGA GTGGTCTCAG GGACAGGAAG TCCTTGGACT AATAGGAGAG  - #               10350                                                                         - - GCCCCATTCA GTAGAGGGAG GAGATTGGCT CCTTAACAGG ATTCAACATG  - #               10400                                                                         - - ATGACACTGA GTGACAATGA GGGGACCCCT CTCAGAAAGA AGAGGGTCAC  - #               10450                                                                         - - ATAGAACCTT GTCCCTATTG TCAGACCTGA GACCTGGACA TGATGACATG  - #               10500                                                                         - - ATGTATCATA CTCACTTCTT CCCAGGTAAT CTCGCGGAGG TGAAAAATAT  - #               10550                                                                         - - TAACAAAGTG GGCAGCCTCA GGTCAGTAGA GAGAAGATTT CTAGGTTGTT  - #               10600                                                                         - - GCAGAAGTAA ATGTGAGGAC CCTGAAGACT GAGGTGAACA CCTACCCCAT  - #               10650                                                                         - - AACAGTGAGG GTAACACAGA TTCCTTCCAC TACTGTTAGC CTAGGGAGAA  - #               10700                                                                         - - CATGAGCAGT TATGGCAATA TGAAGCAAAT TTTACTTCTT CTTAGGGTGT  - #               10750                                                                         - - ACCAGGAAAT TGGTCTTCAA TTCCCTTCAT ATGGGGAGAC AGGCCTCAAG  - #               10800                                                                         - - TCAAGACAGG GAGGATTTGC ACAGGGTGTG ACAGGAATAA GGATGAGGAA  - #               10850                                                                         - - ACTGAAGAGT ATGGGACCAC AGACCCCACA TCAGTAGGAG CCACACAGAA  - #               10900                                                                         - - TCCTCCCTAC TGTCAGCCCT CAGAGACCCC AAGCAGAAAT GTCAGGTGGA  - #               10950                                                                         - - GTTTCCCATC AGGTCCTACT CAAGGGTAAC AAGGGAAGTG AGGGTCTTGA  - #               11000                                                                         - - TCTTAGGGTG GTAGTCTCAC ATCAACAGAA AAAGAAATCT TAGACCTGTC  - #               11050                                                                         - - CAGAAGTTAA AAATTTAGGA CCCTGAGTTG GGACCCTGCA TGGAACTACC  - #               11100                                                                         - - CATGATAGGG CCTTGAACTG CCTGCCACAG GTTTCATTCT TGTGAGACCA  - #               11150                                                                         - - TTGGCAGGTA TATAGCCAGA TGAGGCCATC CTTGCTTCCT CTTGCTGGAT  - #               11200                                                                         - - CTTAAGAAGG TGTGGGCCTC ACTTTGAGGA GATGTCATCA GGTCAAGAAG  - #               11250                                                                         - - GAGGGGATCC CCAGGCCCTT CCAGGAGTAA AATCAGGGAA TCTGAGTGGA  - #               11300                                                                         - - GACTGATGGT ACAACATAGC CTTGAATAGA GGGGACAACA AAGTGTCATG  - #               11350                                                                         - - CCCTACCCTT TGATATCAGC CTTAGAAGAC TCAGAGCAGG GCTGTCAGGG  - #               11400                                                                         - - GAGGCACCTC ATCACTTCTT TATATACGGT CTAGGGGAGG GGAAATCTTT  - #               11450                                                                         - - GGTCTGAAAG TTCAGCTACC AGTCAGCAGA AGAGATCCAA TACATGCCAG  - #               11500                                                                         - - TCACTGTGCC AAGTGCTTTC CATACATTAC CCACATCCTG CCAGTTCTGC  - #               11550                                                                         - - CAGACAGGGC TTACCAAACC CATTCCATAG ATGCAGAACC TAAGGCTCAT  - #               11600                                                                         - - AGGATTTAGT AATGTGCCCA AGTCACACTG CTAGTAATGA CAGGGCTGGG  - #               11650                                                                         - - ATGAGAACAC TGGTCTGAGT TAGTCAAAAG CCCACACTGT GCCACTCCTC  - #               11700                                                                         - - TCAGACAAAG GATGACCTTG TTCCTTAATT AACTTTTCCT CTCGTTACTG  - #               11750                                                                         - - CAAAATGTAC CAGAGGCAAT AAAAAGAAAG ACTGTGGCCA AACGATGGAC  - #               11800                                                                         - - TGTAATATAT AGCCAGAACA ATAAAAATAT CAAAATAAAT GGAAGGTTTG  - #               11850                                                                         - - AGTAAGGAAA AAAATAATAT TTTGATGACA TAATTTACAT ACATATGTGA  - #               11900                                                                         - - TGTCCAATAA ATTCAAAAAA TGAGGAGGTA CAAAAAAATC ATCTGAGCCA  - #               11950                                                                         - - CTTCCTTAGA AAAAGTAAAT ATATCACAAC AGAAGTTACA TGAGACCCTA  - #               12000                                                                         - - AATCTGGCTG GGCAAAAATA GGAGATGGAC ATTTGCTTTT TCTCTTATAG  - #               12050                                                                         - - CCTACCACCT ATCCTGGCCC CCTGCCTTGT GCTGTAGATT TCCCATATTG  - #               12100                                                                         - - CATCACTCCT AGGGCAGGGG CCTGTTCTCT GAAAGGTTTG CAGAGAGGAC  - #               12150                                                                         - - ACTGCTCAAG AGTTTCGCAG AGATGTGGCA TTTCCCAGGA AGCCTTTAAT  - #               12200                                                                         - - CCCACTGCAA GAGCCTGTAT GAGGACATAC ATAAATCAGG ACTGGAAAAA  - #               12250                                                                         - - CAAACACTGC AGAATATTGG TTATTACCAA AAGACTATCC CCTGCCTGCT  - #               12300                                                                         - - TTCAGACCTA GAGGGTCCCA GGCAGAACTG TCAGGATGAG CATCCTCTCA  - #               12350                                                                         - - TTTATTTTTC ACAGTTGTCA GGAACTTGAG AGCTTTAGCC TAAGGAGGGC  - #               12400                                                                         - - AAGCTTAGGT CAGGAGAGAG ATAAGACCCA AGATCTGTTA GATACAAATG  - #               12450                                                                         - - CCAGATCCCT TAAAAGTAAT TAAGAAAGCA CCCATCCAGA ACACCGGGGT  - #               12500                                                                         - - CCCGAAGAGT TCAGGCCCTG CTGTTGACCC TTGGAGGTCC TGAATTCAAT  - #               12550                                                                         - - GGTTGGATGA AATATAGAGA GGCCTTGAAA GATGAAGCAG AGAGAGCCTG  - #               12600                                                                         - - AGCCAGAGCT GCAGCCAGGG CTGTGCTACT GCCATGGCCA TGGCACATAC  - #               12650                                                                         - - CAGAGCCACG TCCAGCTGCT CCTCCTACAT CTAGTGAGAT CTGAGACAGA  - #               12700                                                                         - - TTCTTCACTT TGTAGTTGAA AAGATAAGTC AACATTCTAA GTAGTGGAGA  - #               12750                                                                         - - GTCAATTTTG ACCTAGGGCA AACATATTGT ATGTCTTATT TTTGTTTTTG  - #               12800                                                                         - - CTCTACTTGA ATAATTGGAA GATGTATCTT TTTTATTTTT GGTACTTTTA  - #               12850                                                                         - - AAATGTATTC ATTTTAATAG AAGATTTATT TAGCTTCAGT ATCTGTGTTT  - #               12900                                                                         - - ATGAATAACA TGGATAACAT ATTTATTTCT GTTTTCCATA TATAAATGTA  - #               12950                                                                         - - AAAGTGCTGG TATTTTTTTA TCAACAAACT GAAAATCCTT AGGTCTCTCT  - #               13000                                                                         - - TTGTGGTCCA GAACAAGATA ACATAGCATA TGAATAAGGA CTTTTTTATT  - #               13050                                                                         - - GTATAAATTT AAGTTGTACA GTAGGACGTT TTGATATACA TATATGGCCA  - #               13100                                                                         - - TGCACCACAT GATGATGTCT CAATCAGGAC CACATATACA ATGGCAGTCT  - #               13150                                                                         - - CGTAAGATTA TAATGGAGCT AAAAAACTGT ATTGTTTACA TGTGTGTGGT  - #               13200                                                                         - - GATGCTGGTG AAAACAAACC TACTGCACTA TCAGGCCTAT AAAAGTATAG  - #               13250                                                                         - - CACATACAAT TATGTGCAGT ACATAGCGCT TGATACTGAT AATAAACAAC  - #               13300                                                                         - - AATGTTACTG GTTTATATCT TTATTATACT ATAGTTTTTT TTAAATCAGG  - #               13350                                                                         - - TTGGTGCAAA AGTAATTACA TTTTTTGCCA TTAAAAGTAA TAAGAATTGA  - #               13400                                                                         - - GATGGGGTAG TAGTAGTAAT AATATTCTAG TAATACTAAA TAAGTAGTAA  - #               13450                                                                         - - TATTAATAGG TAGTACTATT AAAATATTAA TATTAATTAA TAAGTAGTAA  - #               13500                                                                         - - TATTAAAAGT AGTAATAATT GGGACGGAGT CTTCCTCTGT TGTCCAGGCT  - #               13550                                                                         - - GGGGTGCAGT GGTGCAATCT CAGCTCATTG CAACCTCTGC CTCCTGGATT  - #               13600                                                                         - - CAAGCATTCT CCTACCTCAG CCACCTGAGT AGCTGGGATT ACAGGGGCCT  - #               13650                                                                         - - GCCACCACGA CCAGATAATT TTTGGACTTT TAGTAGAGAT GGAATTTCAC  - #               13700                                                                         - - CATGTTGGTC AAGCTGGTCT TGAACTCCTG ACCTCAAGTT ATCCACCCGC  - #               13750                                                                         - - CTCATCCTCC CAAAGTGCTG GGATTACAGG TGGTACTTTT ATTATTATTT  - #               13800                                                                         - - TAGAGTGCAC TCCTTCTACT AAAAAAAAAA ATGTTAACTG TAAAAACAGT  - #               13850                                                                         - - CTCAGGCAGG TCCTTCAGGT TGTATTCCAG AAGAAGGTGG TGTTATCATA  - #               13900                                                                         - - AGAGATGACA GCTCCATGCG TATTATTGTC CCTGAAGACC TTCCAGTGGG  - #               13950                                                                         - - ACAAGATGTG GAGGTAGAAG ACAGTGATAT TGATGATCCT GACCTTGTGT  - #               14000                                                                         - - AGGCCTAGGC TAATGTGGGT GCTTTTGTCT TCATTTTTAA CAAAAACGTT  - #               14050                                                                         - - TAAAAATTTA AAAAGTAAAA ATAGAAAAAA GCTTATAAAA TAAGAATATA  - #               14100                                                                         - - AAGAAAGAAA ATATTTTTGT ACAGCTGTAC AATGTGCTTA TGTTTTAAGC  - #               14150                                                                         - - TAAGTGTTAC TACAAAAGAG TCAAAAAGCT AAAAAAAATT AAGAAGTTTA  - #               14200                                                                         - - TAAAGCAAAA AAAGTTACAG TAAGCTAAGG TTAATTTATT GTTAAAGAAA  - #               14250                                                                         - - GAAAACTATG CTTGAGAATT TAGTGTAGTC TAACTGTACA ATGTTTATTA  - #               14300                                                                         - - AGTCTATAAT AGTGTACAGT AATGTCCCAG GCCCTCACAT TCACTCACTA  - #               14350                                                                         - - TTCAATCACT GGCTCACCCA AAGCAACTTC CAGTTCTGCA AGCTACATTA  - #               14400                                                                         - - ATGGTAAATA CTCCACACAG GTGTATGATT TTTAACAATC TTTTGTACCA  - #               14450                                                                         - - TATTTTTTAC TGTACCTTTT CTGTGTTTAG GTACACAAAT ACTTAGCACC  - #               14500                                                                         - - GTGTTACATT TGCCTATAGT TTTCAGTGCA GTCACATGCT GCACAGGTTT  - #               14550                                                                         - - GTGGCCTAGG AGCAATAGGC TACACCAAAT AGCCTACGTG TGTAGTAGGC  - #               14600                                                                         - - TATATACCAC CTAGGTTTAT GTAAGTATAC TCTATTATAT TTGCACAACA  - #               14650                                                                         - - ATGAAATTGC CTAACAACGC GTTTCTCAGA AAGTATCTTC ATTGTTAAGC  - #               14700                                                                         - - AATGCATTAC AGTACATGGT GAAATGCTTA CTACAGGTAA GCAATTTAAT  - #               14750                                                                         - - ATATCCATTA TATCATATAG TTACCTTGTT TTGTGGTAAG AGCAGCTAAA  - #               14800                                                                         - - ATCTACTCTT AGAAAATTTG CAGTATGCAA TACAATATTA CTAACTGTAG  - #               14850                                                                         - - TCCTCATACT GTACTTTAGA TCTCTAGATT TATTCGTCTT ATAAAACTGC  - #               14900                                                                         - - AACTTTGTAC TGTTTGACAT GCATCTCTGC ATCCCCTCCC CACCCTGCCT  - #               14950                                                                         - - CTGGTAACTA CTGTTTTATT CTCTTTTTCT ATGTATTTAA CATTTTTTTC  - #               15000                                                                         - - TTTTTCGATT TTACATATAA GGGATATTAA TAGCATGCAG CATTTTTCTT  - #               15050                                                                         - - TCTGTGTCTG GTTTATTTCA TTCAGCATAA CGTCCCCCAA GTTCATCTGT  - #               15100                                                                         - - GTTGTTGCAA TGGCAGAATC TCTTTCTTTT TCAAGGCTAA ATAACATTTT  - #               15150                                                                         - - TTTAAATTTT ATCTTAGTTT CAGGGGTACA TGTGCAGGTT GTTATATAGG  - #               15200                                                                         - - CAATTGCATG TCATGGGGGT CTGGCGTACA GATTATTTCA TCACCCAGGT  - #               15250                                                                         - - AATAAGCATA GTACACAATA GGCAGTTTTT CCATCCTCAC CCTACTCCCA  - #               15300                                                                         - - CCTTCCGCCC TTAAGTAGGC CCCAGTGTCT GTTGTTCCCT TCTTTGTGTC  - #               15350                                                                         - - CATGTGTACT CAATGTTTAA CTCCCACAAA GAACATGCAG TATTTGGTTT  - #               15400                                                                         - - TCTGTTTCAT TGTACATATA TACCACAATT CCTTTATCCA TTCATCCATT  - #               15450                                                                         - - GGTAAACAAT TTTGTTGTTG CCGCATCTTG GTCATTGAGA ATAATACTGC  - #               15500                                                                         - - AGTGAACATG GGGGCATAAA TATCTACAGG AGGTGATGAT TTCGTTTCCT  - #               15550                                                                         - - TATGCCCAGA CAAGGGATTG CTGGGTCATA TGGTAGTCCA TTTTCAATTT  - #               15600                                                                         - - TTTGAGAAAG CTCATACTGT ATTCTAGAAT GGCTGTACCA ATTTGCATTT  - #               15650                                                                         - - CCACCAATAG TGTAGAAGCG TTCCATTTTC TCTACACTCT TGCCAACATT  - #               15700                                                                         - - CATCTCCAGT GGGTTTTTGT TTGGTTGTTT TTGTTTTATT TTTTTATAAT  - #               15750                                                                         - - AGCCTTCCTA ACTTGTGTGA GGTGATATCT CGCTGTGGTT TTGATTTGCA  - #               15800                                                                         - - TTTCCCTGAT GGTTAGTGAT GTTGAGCACA TCTTTGTATA GCTATTGGAC  - #               15850                                                                         - - ATTTTTATGT CTTCTTTGAG AAATGTCATA CAAGTAGTGA ATTTTCTTGA  - #               15900                                                                         - - GTACTGATTA TGTTGGAAAT GTGATTGAAT ACTGTATTAA AAATTGTTGA  - #               15950                                                                         - - GATCAAAAAA TTTTTTTAAA AAAGGTTGTC TATTCTTGGT TTGCCTAATT  - #               16000                                                                         - - CCTTTTAGTC TTTCTTCTTA TAAAATTAAG AGTTACATAT CTGGATTTGC  - #               16050                                                                         - - TTAGATTATT AAAGAATGTA GGAGAGATTA AATCCTAATT TATTGGACTT  - #               16100                                                                         - - CATACTCACT CTCTTGTTTA TTCCTTAATC ATTAATTGAG CATCAGCTCT  - #               16150                                                                         - - TTGGAAGTCT TCATGTTAGT ACTGGGAATG TTTTCCCCAA AACAAATCAA  - #               16200                                                                         - - TTTCTTGCCC ATTTAATTTT AGAGTCTGGG AGCAGCTGTC ATAAAAAAAA  - #               16250                                                                         - - AAAAGATGAT GAGGTGCTCT CTAAAACTTA AAGAACAAGT ACAAAGGAGG  - #               16300                                                                         - - AATGAGAAAA AGAGGAGGTT TGAGATGAGA GCAATCAAGT GTAAATGCCC  - #               16350                                                                         - - TGAGGCAAGG CAGTTTGGAG TGTTAGGAAA CCTCAAGTCC CCCATTGGGA  - #               16400                                                                         - - GGTAATTTTA AGGGAAACTA CATGGTGGGC TGGATGAGAC TGTGGGAGGG  - #               16450                                                                         - - GCCAGGCCCT CAGATGGTGC CTCTCAGAGG TGTGAGACAA AGCCTGGAAT  - #               16500                                                                         - - GGGAAGTAGT TCTTAACAGT TATTTTGTGC TCACGGATAA ACCAGAGAGA  - #               16550                                                                         - - ATCTGCACCT GGGGCAGGAA TGGAATGTGC CCTGTGCTCT TGTCCCAGTG  - #               16600                                                                         - - CAGCAGAACA TAGTCACAGT GCACAAACTA GGTGCTTTAT ACAGTTGGTG  - #               16650                                                                         - - CATTCAGTGT TGAGAGACTA AGCCCGGAAT GGGAAACTAT CCCTAACATT  - #               16700                                                                         - - ATCACTTTCC TGTTGTTGGA AAACCAGAGA GAACCCCTAC CTAGGACAAA  - #               16750                                                                         - - AGTGAAAAGT GTTCTATGTC CCTATCCTAG CACAGTCTAA TACAGTGCAC  - #               16800                                                                         - - AACCTAGGTG TTCTATGTAC ATCATCTCTA GTGAGTTTCT GAGAAATAAG  - #               16850                                                                         - - GGAGATGACA GCTTCAGGGG AGGTAAGATG CCCAGAAGCC ACCGTGCTGG  - #               16900                                                                         - - CACTCTTTGT CCTGGGTTGG AGAATCAAGA GCCCGCTCTA TTAAAAGGAC  - #               16950                                                                         - - TTTCAACAGG GGTGCAGCCA GGCATGGTGG TTCATGCCTG TAATCCCAGC  - #               17000                                                                         - - ACTTTGGGAG GCGAGGCAGG TGGATCTCTT GAGATCAGGA GTTCAAGATT  - #               17050                                                                         - - AGTCTGGCCA ACGTGGTGAA ACCCCGTCTC TACCAAAAAT ACAAAAATTA  - #               17100                                                                         - - GTTGGGCGTG GTGGTGGACA TCTGTAATCC CAGCTACTAG GGAGGCTGAG  - #               17150                                                                         - - GCACGAGAAT CACTTGAGCC CTGAGGCACA GGTTGCAATG AGAAGAGATA  - #               17200                                                                         - - GTGCCTCTGC ACTCCAGCCT GGGTGACAGA GTGAGACTCC ATCTCAAAAA  - #               17250                                                                         - - AAATAAATAA ATAAATAATA AAAATCAAAA CAGGAGTGGT TAACATAGAA  - #               17300                                                                         - - GGGTGCCTAG GAGTTAGAAA AAAATATTGG TCATTGACAA AAATTTTGAG  - #               17350                                                                         - - ACTTGAGTTG TATCCAATTG GAGAAGGCTC TTCCACAAAC ACTTTATAAA  - #               17400                                                                         - - TTATATAATT TTCCTTGCTA AGCAGCATTT TGTTTGATTA TAATTTCTTT  - #               17450                                                                         - - GTTTGGAGTT TGGTAATATG CTCAAGTTCA CAGGGTGAGA AGTGACAGGG  - #               17500                                                                         - - CTGGGACTAG ACTCCTGTTG TGAATTCTTC TAGAGCCCAC ACTCTTCATT  - #               17550                                                                         - - CTGTTCCTCT CTGCCTGGAG CAGACTTTGT GTTCTTTAAT TCACTTTTCT  - #               17600                                                                         - - TCTCAATACT TCCAAATATA TCTCAGGGGT TAAAAAGAAG GATTCTGTGC  - #               17650                                                                         - - CCAAAGAATT GGATTGGAAT ACATAGCCAA AGCAATAAAG AATATCAAAA  - #               17700                                                                         - - TAAATGAAAG CTACAAATAA AGAGGAGAAA GAGATATTAT TCAGTGACAA  - #               17750                                                                         - - TATGATCATC TACATATGCA CAAGTCAGAT AACCTCAAAA TCAGGGACCA  - #               17800                                                                         - - CACAATCATC TTGAACAGCT CCTTCCCCTT AAGAAGTAAA TCAATTAAAA  - #               17850                                                                         - - GAGAAGTCAC ATAAGAGGCT AGTGCTGGCT ATTAAAAGAG TAAATCAGCA  - #               17900                                                                         - - TCATTTTTCT CTGACAACCC ACCAGCTACC TTGGACTCCC TGCTTTGTGT  - #               17950                                                                         - - TATAATTTCT TTACCTCACA TTAGCCCTGG GGCACAGACT TGCAAGGTTT  - #               18000                                                                         - - GCAGTAGGAA CGCTGCCCAA GAGTTTGCAG GGATGTGGCA TTTCCCAGGA  - #               18050                                                                         - - AGCCTTTATT CAGAGAGGCA AGAGAAAATA AGGAGGACAC CCATAAATAA  - #               18100                                                                         - - ATACTGGAAA GACAACCACA CCAAAAGATA ACGGGGCAAT CTACCCAAAC  - #               18150                                                                         - - CTCTTCTCAG CCTAGAATGC CCCAGGCAGT GCAGTCAGAC TGAGCATCCT  - #               18200                                                                         - - CTCTTTTACC CTTCAGAGTT CTCAGTGAAG TGAGAGTTTT GACCTAAGGT  - #               18250                                                                         - - GACCTCAGTT AACTAGAGGG AGGATCCAGG GTCTGGCAGA TATCAGGGAG  - #               18300                                                                         - - ACCTTGAATG AAGAACAGGA GGAGCCAGAA CAGTGATGTC CATAGAAACC  - #               18350                                                                         - - CGACTCCGCT GTCTGCCCTC ACAGGACCCA AACATCCCTG GCCTAATGTG  - #               18400                                                                         - - GCTCATCAAA ACTTCAGCCT AGATCTCGGA GGACCTTGAT CTGAGCAGGA  - #               18450                                                                         - - TTCATGGCAT GGAATGTGGA GTCCAGGACT GGCCAGCAGC GAAGTGAGGT  - #               18500                                                                         - - TCTTGAGTGA GTAAAAAGGG AAAACTAAAC CCACAAATAA GGGGACTTCT  - #               18550                                                                         - - CGGAGCCCAA TCCATATTTT TAACCCTGGA AAGCCCTAGG CAGAGCTATA  - #               18600                                                                         - - AAACTGGCTT GCTCTCATTT CAGCCTGGCG ATCTCAGGGA GGGGAAGGCT  - #               18650                                                                         - - TTGTCTAACA GGGCAGCCTC AGTTCTTCAG AGGTCTCTTG GCCCTAACTA  - #               18700                                                                         - - GAGTCAAGAT GAAGACCAAG CCGGTGCGCA TCTCAGGCCT GTAATCCCAG  - #               18750                                                                         - - CACACTGGGA GGCCGAGGCG AGTGAACTGT TTGGGCCCCC CAGGAGTTCG  - #               18800                                                                         - - AAACCAGCCG GGACAACATG GCGAAAACCT GTCTCTACAA AAAAAAAAAA  - #               18850                                                                         - - ATGGCGGGCA GCAGTGGAGC ACCCTGTAGT CCAGCTACCA GGAGGCTGAG  - #               18900                                                                         - - GTGGGAGGAT CGCTTAAGCC CTAGGGGTCA AGGCTGCAGT GAGCCAAGGT  - #               18950                                                                         - - CACGCCACTG CGCTCCAGCC TGGGTGACAG AGAGAAACAC TGTCTCAAAT  - #               19000                                                                         - - TAGCCGGGCG TGGTGCGGCG CACGTGTAGT CCCAGCTATT CGGGAGGCGG  - #               19050                                                                         - - AGGCAGGAGA ATCGCTTGAA CCCGGGAGGC AGAGGTTGCA GTGAGCCAAG  - #               19100                                                                         - - ATAGCGCCAC TGCACTCCCG CCTGGGTGAC AGAGCGAGAC TCCATCTCAA  - #               19150                                                                         - - AATAAATAAA TAAATAAATA TAAACTTTTT TTTTTTAATT TTAAGGCAGG  - #               19200                                                                         - - GCTCTGGCTC ACGCTTGTAA TCCCAACATT TTGGGAGGAC GAAGCCAGCC  - #               19250                                                                         - - TCATCGCTTA AGCACAGGAG TTCGAGGACT GGGAGGTGGA GGTTGTGGTA  - #               19300                                                                         - - AGTCAAGATT GCGCGGCTAC ACTCCAGCCT GGAAGACAGA GAGAGCCCCT  - #               19350                                                                         - - GTCACAAAAA AAAAAAAAAA AAAAAAAAAA AAGAAAGAAA GAAAGAAAGA  - #               19400                                                                         - - AATATGAGGC CCACCGGTGC TAACCAGGGA ACCTCTCTCC AAAAGAAGGG  - #               19450                                                                         - - CCCACCAAAA GCCCTAACCC TGTTTCAGGT CTTTGAAGCC CCAGGACATG  - #               19500                                                                         - - GTCCGATAAC ATGCCTAGAC TTCCCCTCTA GGGACTATGG GAGGGGAGGA  - #               19550                                                                         - - TTTTGGAGGT TGGCGGACTT CGCTCAGTAG AGGTGTTACT CTGCTCCGCT  - #               19600                                                                         - - TAGTATCAAG GTGAGAACCC TGAATAAGGA CCTAGGGACC ACTGACTCCA  - #               19650                                                                         - - GAACAGTGGG GTCCCAGCGT GTCACCCGCT GCTGTCAGCC CTCGGAGACC  - #               19700                                                                         - - CCGAGCGGGG TGTGGCTCAG CCTCACTTCC GCTTTGAAAG TGAGGCAGTT  - #               19750                                                                         - - GGCTGACGGG TGCAATAGCT TCAGTCAGGT TCGTGGCCTA GCGTGAGTCT  - #               19800                                                                         - - TAGAACTGAT CCGGAGTAAA GGTAAGAACC CTCAGCGGGG ACTGAAGGGA  - #               19850                                                                         - - CAATCCATGT TTTTAACCCT GGAAAGCCCT AGGCAGAGCT ATAAAACTGG  - #               19900                                                                         - - CTTGCCTCTC ATTTCAGCTG GCGGGTCTCA GGGAGGGGAA GGCTTTCTCT  - #               19950                                                                         - - AACAGGGCAG CCTCAGTTCT TCAGAGGTCT CTTGGCCCTA ACTAGAGTCA  - #               20000                                                                         - - AGATGAAGAC CAAGCCGGTA CGGCGTCTCA GGCCTGTAAT CCCAGCACAC  - #               20050                                                                         - - TGGGAGGCCG AGGCGAGTGA ACTGTTTGGG CCCCCCCAGG AGTTCGAAAC  - #               20100                                                                         - - CAGCCGGGAC AACATGGCGA AAACCTGTCT CTACAAAAAA AAAAAAAAAA  - #               20150                                                                         - - TGGCGGGGCA GCAGTGGAGC ACCCCTGTAG TCCCAGCTAC CCAGGAGGCT  - #               20200                                                                         - - GAGGTGGGAG GATCGCTTAA GCCCTAGGGG TCAAGGCTGC AGTGAGCCAA  - #               20250                                                                         - - GGTCACGCCA CTGCGCTCCA GCCTGGGTGA CAGAGAGAAA CACTGTCTCA  - #               20300                                                                         - - AATTAGCCGG GCGTGGTGCG GCGCACGTGT AGTCCCAGCT ATTCGGGAGG  - #               20350                                                                         - - CGGAGGCAGG AGAATCGCTT GAACCCGGGA GGCAGAGGTT GCAGTGAGCC  - #               20400                                                                         - - AAGATTGCGC CACTGCACTC CCGCCTGGGT GACAGAGCGA GACTCCATCT  - #               20450                                                                         - - CAAAATAAGT AAATAAATAA ATAAATATAA ACTTTTTTTA TTTTTTATTT  - #               20500                                                                         - - TAAGGCAGGG CTGTGGCTCA CGCTTGTAAT CCCAACATTT TGGGCGGAGG  - #               20550                                                                         - - AAGCCAGCCT CATCGCTTAA GCACAGGAGT TCGCGGACTG GGAGGTGAGC  - #               20600                                                                         - - TGCCCCTGTA GTCCCAGCTA CCCAGGAGGC TGAGGTGGGA GGATCGCTTA  - #               20650                                                                         - - AGCCCTAGGA GTCAAGGCTG CAGTGAGCCA AGGTCACGCC ACTGCGCTCC  - #               20700                                                                         - - AGCCTGGGCG ACAGAGAGAG ACACTGTCTC AAATTAGCCG GGCGTGGTGG  - #               20750                                                                         - - GGCACAGGTG TAGTCCCAGC TATTCGGGAG GTGGAGGCAG GAGAATCCCT  - #               20800                                                                         - - TGAACCCGGG AGGCAGAGGT TGCAGTGAGC CAAGATCGCG CCACTGCACT  - #               20850                                                                         - - CCCGCCTGGG TGACAGAGCG AGACTCCATC TCAAAATAAA TAAATAAATA  - #               20900                                                                         - - AATAAATAGA TATAAACTTT TTTTTTTTTT TAATTTTAAG GCAGGGCTCT  - #               20950                                                                         - - GGCTCACGCT TGTAATCCCA ACATTTTGGG AGGACGAAGC CAGCCTCATC  - #               21000                                                                         - - GCTTAAGCAC AGGAGTTCGA GGACTGGGAG GTGGAGGTTG TGGTAAGTCA  - #               21050                                                                         - - AGATTGCGCG GCTACACTCC AGCCTGGAAG ACAGAGAGAG CCCCTGTCAC  - #               21100                                                                         - - AAAAAAAAAA AAAAAAAAAA AAAAGAAAGA AAGAAAGAAA TATGAGGCCC  - #               21150                                                                         - - ACCGGTGCTA ACCAGGGAAC CTCTCTCCAA AAGAAGGGCC CACCAAAAGC  - #               21200                                                                         - - CCTAACCCTG TTTCAGGTCT TTGAAGCCCC AGGACATGGT CCGATAACAT  - #               21250                                                                         - - GCCTAGACTT CCCCTCTAGG GACTATGGGA GGGGAGGATT TTGGAGGTTG  - #               21300                                                                         - - GCGGACTTCG CTCAGTAGAG GTGTTACTCT GCTCCGCTTA GTAACAAGGT  - #               21350                                                                         - - GAGAACCCTG AATAAGGACC TAGGGACCAC TGACTCCAGA ACAGTGGGGT  - #               21400                                                                         - - CCCAGCGTGT CACCCCCTGC TGTCAGCCCT CGGAGACCCA GAGCGGGACG  - #               21450                                                                         - - TGTCTCACCC TCACAACTCC CTGCCCCTTA CAAAAGGGGA CCCACACGTC  - #               21500                                                                         - - TCACCCTTGT GGTTGACCCT GGGAGGTCCT GTTTGAGTTC TGTCTGGAAA  - #               21550                                                                         - - GGCACCCAGA GGGAGGGTCT TTCACTAAGG GAGCAGCCCC CAGTTATTCA  - #               21600                                                                         - - GTTGGCGGGG ACCTGGACCC TAACTGGAGC CAAGGTGAAG TCTCCGAGTG  - #               21650                                                                         - - CTAAAGGATG GGATCTGTTC CCAGTAGGGG CGTCAACAGA AAGCAGCAGC  - #               21700                                                                         - - AGCCCTACTG GTTAGCATCC TCCTGGTTAG CACCAGTGGT CCTTGTCTTT  - #               21750                                                                         - - TTTATTTATT TTTATTTTTT TTCAGACAAG GTCTCACTGC TGCTAGCCAG  - #               21800                                                                         - - TTTAGAAGGC TCCAGACAAG TGTAGTCATA AGCGGAGGCC CTGACTTCCC  - #               21850                                                                         - - CATCCAGGGA GGGAAGTGTT CAGGGAGATG AGGGTTTTGT TTGGAGGTTG  - #               21900                                                                         - - GCGAACTCAG GTCAGTAGAG GAAGAAATTT CAGGCTGTGA TAAGATACCA  - #               21950                                                                         - - AGGTGAAGAC CCCTGAAATG AGAATCTAGG GACCAGCAAC TCCAAAACAG  - #               22000                                                                         - - TGAAGTCTCA TAGAGTTCCA CCCGTGTTGT CAGCCATCAG ACCCCAGGAA  - #               22050                                                                         - - GCTGTGAACA GATAAGGCTC TTCCTCACTT CCTTGGAAGT GCTTTGAAGG  - #               22100                                                                         - - GGAGGATCTG GAGGCGAGGG GCACGGGATC TCTTCGGCAG AGGGTGAATT  - #               22150                                                                         - - CCTTGGACTG TCTGGAGTCA AGGTCAGGAC CCTGAATGTG CATGAAAGGG  - #               22200                                                                         - - ACCACCATCC CCCAACCTGT AACAAAGAGG GCCACACTAA ATCCTGCCCC  - #               22250                                                                         - - GGAAGTCTTC CCTGGGAATA CCTTGAAAGC TGTCTGACAG ATATCTACCT  - #               22300                                                                         - - GGAAAGTCTC AGGGAGGGAA GGGCCTTGGT CTAAGAAAGT AGCCCCAGTT  - #               22350                                                                         - - CAGCAGGTGG AAGAGAGACT TGGGTCCTAA CTGGAGTCAA GGTGAGGGCC  - #               22400                                                                         - - CTGAGTGCTA ATGAAGCAAT CTCTCTGCAA TAGAGGTGTC AACACAAACA  - #               22450                                                                         - - GTGTCCTTGC ACTCATTTTG CAACCTCCAG GCAAAGGTAT TCATAGGTGA  - #               22500                                                                         - - GGGGTCCCTG ACTTCCCTGT CTAGGGTCTT GTTCTGAGGC TGGAGGACTT  - #               22550                                                                         - - AGGTTAATGG AGGGAAGTGT CCCACATCCT ACTAAGAGTC AAGTCAGAGA  - #               22600                                                                         - - CCTGAGAGAA AACTAAAGGG AACACTCTCT CTAAAAAGTA GAGTCCCACA  - #               22650                                                                         - - AATTGTGCCT CTTCTCTCAG CTCCAGGAAG CTTTGGAATA ACGTCAGCCC  - #               22700                                                                         - - CCCTTACTGC CTAGAGAGTG CCAGAAAGGT TGAACTAATT ACATGCCCCA  - #               22750                                                                         - - GTTCTGCAGA GGGAAGAGTG AGGAGACCCA GAGCCTTACA GGGGTCACAG  - #               22800                                                                         - - TGAGGACCCT GAATGAAGAC TAGTGATACA ACTCCTCCCA CATAAAGAAG  - #               22850                                                                         - - AGACAACAAA GATTCCCCCA CTCCCCACTT GCAGCGCTGA TGAGGCTGGG  - #               22900                                                                         - - CATGGAGGTC AGGTGTATGT GAACTCACCT TCTTTTAATG GCATTAGGTT  - #               22950                                                                         - - GCTGAGAGCC TTTATCTAAA GTGAGGGGCA TCAGAGCAGC AGAGGCAGCT  - #               23000                                                                         - - GTCCTAGGTC CTATCTAGAG ACAAGATGAG GACTATGAAT GAGGTGTCAG  - #               23050                                                                         - - GACTCCAATA AGCCCAGGAA AGAGTAGGAC TCCACAATGC TGTTAGCACC  - #               23100                                                                         - - CAGTTCCTCC TGTCAGGGGT GGTAAGGCTG AGACATTCCT TCACCTCCTC  - #               23150                                                                         - - TTAGGTGGTC CCAAGGAGAT GAGGACATTT GCTGGAGGTG TCAAATTTAG  - #               23200                                                                         - - TGCAGCACAG GGGAGAAGAC CCAGCCCTGA CAGTTTTTAT GATGGTCCTG  - #               23250                                                                         - - AGTGTGAACT GAGAGAAACC TCCTACCCCA GAGTAAAAGG AGATCCACAA  - #               23300                                                                         - - GGACTAGACA TGCCACGGCT GCTCTCAGTC CCAGTATACA CAGAACAGGG  - #               23350                                                                         - - CTGGCAGGCT GTGGCCTAAG GCACACTTTA ATTACTTTCA CAGGGTTCTC  - #               23400                                                                         - - AGAGGACAAA CTGATCAGAA CAGAAGCCTC TGGGTTTCCA GAGCAGTGCT  - #               23450                                                                         - - CTCACAGAAA ACTGCAGAGG CGACCTTCTT TTAAATCCAA AGTGGTACCT  - #               23500                                                                         - - CTCTGCTGAA GGCACTCATA CCCTCTCTTT CTCTCTCTCC TCCAGGTGCC  - #               23550                                                                         - - TGTATCACCT GCCCTTCTGC TGACACTCCT GCCTGCTGTT CCTGACTACA  - #               23600                                                                         - - GCCATCATGC CTCGGGGTCA GAAGAGTACG CTCCATGCAC GTGAGAAACG  - #               23650                                                                         - - CCAGCAGACC CGGGGTCAGA CCCAGGATCA CCAGGGTGCT CAGATCACTG  - #               23700                                                                         - - CAACTAACAA GAAAAAAGTA TCCTTTTCAT CCCCTCTTAT TTTGGGGGCT  - #               23750                                                                         - - ACTATCCAGA AAAAGTCTGC TGGTAGGTCA CGTAGTGCTC TCAAGAAGCC  - #               23800                                                                         - - TCAGAGAGCA CTATCCACCA CTACATCTGT AGATGTTTCT TACAAAAAGT  - #               23850                                                                         - - CATACAAGGG AGCCAACAGC AAAATTGAGA AAAAGCAAAG CTTCTCTCAG  - #               23900                                                                         - - GGTCTATCCT CCACTGTGCA GTCTCACACA GACCCTCTAA CCATGAAGAC  - #               23950                                                                         - - AAATATGTTG GTGCAGTTCC TGATGGAAAT GTACAAGATG AAAAAGCCCA  - #               24000                                                                         - - TTATGAAAGC AGATATGCTA AAAATTGTCC AAAAAAGCCA TAAGAATTGC  - #               24050                                                                         - - TTCCCTGAGA TCCTTAAAAA AGCTTCTTTC AACATGGAGG TGGTGTTTGG  - #               24100                                                                         - - TGTTGATTTA AAGAAAGTTG ATTCTACCAA GGACTCCTAT GTCCTTGTCA  - #               24150                                                                         - - GCAAAATGGA TCTCCCCAAC AATGGGACAG TGACTCGTGG GAGGGGATTT  - #               24200                                                                         - - CCCAAGACAG GTCTCCTGCT GAATCTCCTG GGCGTGATCT TCATGAAGGG  - #               24250                                                                         - - CAACTGTGCC ACTGAGGAGA AGATCTGGGA ATTCCTGAAT AAGATGAGAA  - #               24300                                                                         - - TATATGATGG GAAGAAACAC TTCATATTTG GGGAGCCCAG AAAGCTCATC  - #               24350                                                                         - - ACCCAAGATT TGGTGAAGCT TAAATACCTG GAGTACCGAC AAGTGCCCAA  - #               24400                                                                         - - CAGTAATCCT GCACGCTATG AATTCCTGTG GGGTCCAAGA GCCCATGCTG  - #               24450                                                                         - - AAACCAGCAA GATGAAGGTC CTGGAGTTTT GGGCCAAGGT CAATAAAACT  - #               24500                                                                         - - GTCCCCAGTG CGTTCCAGTT CTGGTATGAA GAGGCTTTGA GAGATGAGGA  - #               24550                                                                         - - AGAAAGAGTC CAAGCTGCAG CTATGCTCAA TGATGGCAGT AGTGCCATGG  - #               24600                                                                         - - GCAGAAAGTG TTCCAAGGCC AAGGCTAGCA GCTCTTCCCA CGCCTAGTGA  - #               24650                                                                         - - AGTTGAAGCA AATTTTGCAT TTTGTGGTTA AAGAGGGCAG TCACTGTTCC  - #               24700                                                                         - - AAGGAGTGAA GGACTGGGTG TTACTGGAGG GAACACACTG TATAATACCT  - #               24750                                                                         - - TTTGTTTCTG TTCTAAATGG ATAATTTGAA GTTTTATCTG TATTTTGGGG  - #               24800                                                                         - - CATATTTTTC AAATGTTCCT TTTATTTAAC ATTGTAATCT AAGTTTAGGA  - #               24850                                                                         - - TTGATACTGG TCACATTTGT TGTTTAAGAG TAAAAATTTT GCTGTTTTGT  - #               24900                                                                         - - AAAACAGATT GAGAAAAATT CGATCTTATT TAGTGATCTG TTGCAAGATA  - #               24950                                                                         - - ACTTGGAATT AGAATAAGCA TTTCCTTGAA AATGTTTAAA AAAAAAAAGT  - #               25000                                                                         - - CAGCAGTAAA ATGTATGGCA TTAAGAAATA GAGAAAGAGT GTAAGATGGT  - #               25050                                                                         - - CAATATTTGG TTTCCTAAAT GCTTTTACTC TGTGTTTTAA GAAAATGAAA  - #               25100                                                                         - - GATAAATAAC CATATATGTC TGGCTTACTT AAGAATGTAG AATTAAATCA  - #               25150                                                                         - - TAATAAATTA GACCTCATGC TGACACACTC ATTCTCCAAG TGTTAATTGA  - #               25200                                                                         - - GCATCTGCTC TTGGAAGGAT CCATGCTAAT ACTGGGAGGG CTAAGAAAAA  - #               25250                                                                         - - GAAGACCTAG CAACTGACCT TAAAATTATA AGGTCGAGAA GCAGCTATCA  - #               25300                                                                         - - TCTAAGGAAA ATGGTGATAT ACACTCTAAG ACCAAAAGGA TACATGATAA  - #               25350                                                                         - - GAAGGGAGGG AGGTGGTTCC ACATGAGAAC AGTCAAGTAT GAATTATCTA  - #               25400                                                                         - - ATCAAGGCGG TGTTGGGCCT AAGGAAAGTG CAGGTCCCTC AATGGGAGTT  - #               25450                                                                         - - AATCTAAGTG AGGCTCCATG GTGGGCTGGA TGAGGCTGTG GGAATGGCGA  - #               25500                                                                         - - GCAGGGGCCA GGCTCTTAGA AGTTGCCTCT CACGCAGTGC GGGCGCCTGT  - #               25550                                                                         - - AGTCCCAGCT ACTCGGGAGG CTGAGGCAGG AGAATGGCGT GAACCTGGGA  - #               25600                                                                         - - GGCAGAGCTT GCAGTGAGCC GAGATTGTGC CACTGCAGTC CGGCCTGGGC  - #               25650                                                                         - - TAAAGAGCGG GATTCCGTCT CAAAAGAAAA AAAAAAAGAA GTTGCCTCTC  - #               25700                                                                         - - AGAGGTGCGA GACAAAGCTT GGAATAGGAA ACAGTTCTTA ACAGTTACTT  - #               25750                                                                         - - TGGGATCATG GATAAATCAG GGAGAATCTG GGGTAGGAAT GTGTCCTGTG  - #               25800                                                                         - - CTCTTGTCCC AGTACAAATA AACACAGCAC ACACTAGGTG TTTTGTGCAC  - #               25850                                                                         - - ATCATCTCCA GCCAGTTTCT GAGAAATAAG GGTAATACCC TCAAGGGACG  - #               25900                                                                         - - GGGGAGGCCC AGAAGCCACT GTGCTGGTAC TCTTTGCCCT GGGCTGGGGG  - #               25950                                                                         - - ATCCAGAGCC TGTTCCATTA AAAGCGCATT CAATTAGGTT ACCTCATTTG  - #               26000                                                                         - - TGATTTGGCA GACTCTAGGC AAGTTCTAGA TTTTGAGAGG TGGTTAAATG  - #               26050                                                                         - - AATCAAAATA AAAGTGGTTT AGATGGAAGG ATAGCTGAGA GAGAGGGAAG  - #               26100                                                                         - - TTTTTGGTCC TTGAAACTCA TTTTAGAATC TGTGTTGCAT CTACTGAGGA  - #               26150                                                                         - - AGTCTACCTC ATACTAATAT TGAATGATCT ATCAAGTAAT GAGGAAATAT  - #               26200                                                                         - - TATTTAGTTT TCCTTTCTAG GTAGCACTTT GTCTGATAGG GCTTTCTTTG  - #               26250                                                                         - - TCTTAGAATA TGGTATAATC TCTGACATCT CCTGCATTGA AAAATAAGTC  - #               26300                                                                         - - CCAGTGTAGT AGATTGCAAC GTCAGGGTAC AAATTAATCA TAATAAGAAC  - #               26350                                                                         - - TGGCACTATT ATATGTCTCA ATACATCTCA AGCACTTTAC ATACATTGTA  - #               26400                                                                         - - TACATTTTAC CAGTCCTACA AAACAGAGCT TATCAAACCC ACTTCACAGA  - #               26450                                                                         - - TAAAAAGCCT GAAGCCCACA GTCTATTAAT ATGTCCAATT TCACATGGCT  - #               26500                                                                         - - GGTAAGTGAC AGGACTGGGA CAAGACCCCC AGTCTGAATT AGTGTAAAGC  - #               26550                                                                         - - ACACTCTGTT CCATGCCTCC CAACCTGAGA CTGACTGTGT CCCCTTATTC  - #               26600                                                                         - - ACTTTTCTTC TCACTACTCC CAAACGTATA TCAAGTGATG AAAAGATTCT  - #               26650                                                                         - - GTACCCAATC TGCTGGACTG AAATACAGAA CCAGAGCAAG GAAATTACCA  - #               26700                                                                         - - AAATGGTCAA GAGGTGTGAA TTAAGGAGAG AAGATAATAT TTGGTGACAA  - #               26750                                                                         - - TAAGATTACA CATGCAAAGG CAGATAAACT CAAAAGTCAT GGGGTCACAT  - #               26800                                                                         - - TCTGGCAATC TGGACTGGCC CCCCTTTTAT AGGAAGGTAA ATCCTTTCCA  - #               26850                                                                         - - AGAGAAATAC ATAAGAGGGT AGTAAAGAGA AGAAATAGGA TCAATGGTTT  - #               26900                                                                         - - TTTTTTTTTT TTTTTTTTCT CTGACAGTAT GACTTCTATC CTCGGCTTCT  - #               26950                                                                         - - GCCTTGTGTT ACAGCTTCCC TATCTTATAT CACTCCTGGG ACAGGGATGC  - #               27000                                                                         - - ATTGTCTGCA AGATTTATTA TAGGGAAACT GATCAAGAAT TTTTAGGGAT  - #               27050                                                                         - - GCGGCATTTC TCAGGAAGTC ATTAACTAAG GGACAAGAGC CAAGATAAAG  - #               27100                                                                         - - ACCTTCATAT AGGAGGACTC AGCAGAACAA CATGCCAGAC AGAGTTCTTA  - #               27150                                                                         - - GCCTGAGCAT CTCCTCACTT ATTCTTCAAG GTTCTCAGGG ACTTGTGAGT  - #               27200                                                                         - - TCCGCATCTC AGGTCAGTAG AGGGAGGAGT GCCATTCTCT GACACATACC  - #               27250                                                                         - - AAGTTGAGGA CCCTGAATGA AGAATGAGGG AAGCACTCAC CCAAATAGCC  - #               27300                                                                         - - TTAACAGGAT GTGGCTGAAC CTGATTTCTA CTCCGGAGGT CTTAAGAAGG  - #               27350                                                                         - - TAAAGACCAT GATCTGAGGC TGGCTGACTC AGGTCCATAG AGGAAGGATT  - #               27400                                                                         - - CCTAATGTGT GCCAGGAGTA AAGTGAGTAC CCTGAAGAGT GCAGGAACCA  - #               27450                                                                         - - CCAAACCCCT ATCAGTGGGA TCCCACAGAA TCCTCCCTAT TGTCACCTCT  - #               27500                                                                         - - GAGAGATTTA GGCATGAATG TCAGAAAGAG GCACCCTCAT ATCCTCAGTA  - #               27550                                                                         - - GTAACAGAGA AGTGAAGGTC TTGTTCTGAG GTGGGCAATC CCAGCTCAGC  - #               27600                                                                         - - AAAGGGAGGA ATATTAAGCC TTCCTGTGAG TGAGATTTAG GATCCTGAGT  - #               27650                                                                         - - TAGGACCTGA GTAGGACAAT CCACCATAAG ACCTCAACCT GTCTACCACA  - #               27700                                                                         - - GCTGCCACTC TTGGGAGACC ATGGTCAGCT GTGCCAGATA AGGCCACCCT  - #               27750                                                                         - - CTTCCTCCTA TCAGATCTCA GGGAGTGCAG CCTTTTTAAA TGGAGAGTCT  - #               27800                                                                         - - TCAGTCAAAA AAGAGGGGAG CCCTAGGATC TGCTAGGAGC CAAATCAAGG  - #               27850                                                                         - - AAACCGAGTG AGAACTGAGG GGACTGCTCA CCTCTGAATA GAGAGAGCAA  - #               27900                                                                         - - CAGAGTCCAC CCCCCTGCAT AACTCTTAAA GTGCCAGGGC AACATGTAAG  - #               27950                                                                         - - GCTGAGGACT CACCATCACT TTTTTTTTTT TAATAATTGA CCTTCTTTTT  - #               28000                                                                         - - TAGAATAGTT TTACGTTCAC AGCAAAACTG AGCAGAAAGG AGAGTCTCAC  - #               28050                                                                         - - AGGCATCCTG TCCTCCCACA GACACAGCCT CCTTCATATC AACCTCCCAA  - #               28100                                                                         - - GCCAGGGTGA TACATTTGTT ACAATTGAGA ATACATGGAC ACATCATTAT  - #               28150                                                                         - - CACCCAATGT CCGTAGTTTA GGATTCATTC CTGTTGCTGC ACATTCTGTG  - #               28200                                                                         - - GTTTTAATAT TTAAAAATGT ATAATGACAT ATGTTACCAC CATAGAATCA  - #               28250                                                                         - - TCCAGAAATT GTTTTACTGC CTTAAAAGTC TCTGTGCCTG ATCTATTTAT  - #               28300                                                                         - - CTCTCACTCT CCCCAACCTG TGGAAACCAC TGAACTTTGT ACTGTTTCCA  - #               28350                                                                         - - TACTTTTGCC TTTTCCAGAA TGCATATAGA TGGAATTATG CAATGTATAG  - #               28400                                                                         - - ACTTTTCAAA TTGGCCTCTT TCACTTTGTA ATATGTATCT AAGTTTCATC  - #               28450                                                                         - - CATGTCTCTT TGTGGCTTGA TAGTTCATTC CCTTTTAGTG CTGAATAATA  - #               28500                                                                         - - TTTTATTCTG TTAATTTATC CACTCTCCTT CTGAAGGACA TATTGCTTGC  - #               28550                                                                         - - TTCCCAGTTT GGGCAATTAT GAATAAAGCT ACTATAAACA TTTGTGTGCA  - #               28600                                                                         - - GGTTTTTTTT GTGGACATAC CGTTTTCAAC TCATCTGGCT AAATAACAAG  - #               28650                                                                         - - GAGCGCACTT GCTAGATCAT ATGATGAAAG TATGCATAGC TTTGTAAGAA  - #               28700                                                                         - - ACTTACAAAC TGTCTTCAAG AGTGGCTGTA TCATTTTTCA TGTCCACCAG  - #               28750                                                                         - - CAATGACAGT GTGCTCCTGT TGCTCCACAT CCTCATCAGT ATTTGGGGTT  - #               28800                                                                         - - ATCAGGGTTT TTGATTTTGA CCATTCTTAT AGGTATGTAG TGGTATCACA  - #               28850                                                                         - - TTATTGTTTT AATTTGCAAG TCTCTAATGA TATGTGATGT TGACCATCAT  - #               28900                                                                         - - GTCATGTACG TATTTGCCTT CCATATATAT TATTTGTTGA GGTGCCTGTT  - #               28950                                                                         - - TAGATCTTTT GTCCATTTTT AATAGGGTTG GTCAGTTCTT ATTGTTCAGT  - #               29000                                                                         - - TTTAAGACAT TTTTGTATGT TTTGGTTAAC AGTCCCTTAT CAGATATGTC  - #               29050                                                                         - - TTTTGAAAAT AATTTTTTCC CAACCTGGGA GTTATCTTAT TCTCTTTGTG  - #               29100                                                                         - - GTATCTTTAG CAGAGCAGAA GTTTTAATTT AGCGAAGTCC AGATTATCAA  - #               29150                                                                         - - TTATTTTCTT TCATAGATGC CCATCACATT TTATACCAGA ACTAGAAAAG  - #               29200                                                                         - - ATGAAATTCT TGGTCTGAAG TTGCAGTTAT CAGTCAGCAG AAGAGACAGT  - #               29250                                                                         - - CCACAACCCT GCTTGGAGTC CAGATGAGGA TCCTGAGTGC AAACTTGGGA  - #               29300                                                                         - - CCTAAAGAGC CCAGGACAGA GAGAGCACTA AATGCTTCTA GGCAGGGGTG  - #               29350                                                                         - - GTGGGTTGAG GGGCCCCTAG ACTTCCCTCA TCTGGGTCCC AGAAAACTAA  - #               29400                                                                         - - AGAGTCAATT TCACAACACC AATAGAGGGA GGCTCAGGCC CTGCCAAGAG  - #               29450                                                                         - - CTGACATGAT AATTCTAAAG GTAATCAGAG TGGATCCTCT CCAAGCCAGA  - #               29500                                                                         - - ACACAGAAAG CCCCACTGCG AGCCTTGTTG TCACCCAGTC AGCCCCAGGC  - #               29550                                                                         - - AGGGTTGGCA AGCTGCAGCC TAAGGCACAT TGTAACTTCC TCAGCTGGCT  - #               29600                                                                         - - TCTCAGGGGA CAGAATGACT AAGAACAATA GCCCAGTGAA TACTTAGAGC  - #               29650                                                                         - - AGTGTTCTCA AGGAATCCTG CAGAGGCGGC TTCTGAAAAG CCAAGGTAGT  - #               29700                                                                         - - ATCTGCCTGC TGAAGGTGTT CTCAGGATTT CATTTGCTCT TCTCCAGGAA  - #               29750                                                                         - - CCACATCACC TGCCCTTCTG CCTACACTCC TGCCTGCTGT GCCTAACCAC  - #               29800                                                                         - - AGCCATCATG CCTCGGGGTC AGAAGAGTAA GCTCCGTGCC CGTGAGAAAC  - #               29850                                                                         - - GCCAGCGGAC CCGTGGTCAG ACCCAGGATC TCAAGGTTGG TCAGCCTACT  - #               29900                                                                         - - GCAGCAGAGA AAGAAGAGTC TCCTTCCTCT TCCTCATCTG TTTTGAGGGA  - #               29950                                                                         - - TACTGCCTCC AGCTCCCTTG CTTTTGGCAT TCCCCAGGAG CCTCAGAGAG  - #               30000                                                                         - - AGCCACCCAC CACCTCTGCT GCTGCAGCTA TGTCATGCAC TGGATCTGAT  - #               30050                                                                         - - AAAGGCGACG AGAGCCAAGA TGAGGAAAAT GCAAGTTCCT CCCAGGCCTC  - #               30100                                                                         - - AACATCCACT GAGAGATCAC TCAAAGATTC TCTAACCAGG AAGACGAAGA  - #               30150                                                                         - - TGTTAGTGCA GTTCCTGCTG TACAAGTATA AAATGAAAGA GCCCACTACA  - #               30200                                                                         - - AAGGCAGAAA TGCTGAAGAT CATCAGCAAA AAGTACAAGG AGCACTTCCC  - #               30250                                                                         - - TGAGATCTTC AGGAAAGTCT CTCAGCGCAC GGAGCTGGTC TTTGGCCTTG  - #               30300                                                                         - - CCTTGAAGGA GGTCAACCCC ACCACTCACT CCTACATCCT CGTCAGCATG  - #               30350                                                                         - - CTAGGCCCCA ACGATGGAAA CCAGAGCAGT GCCTGGACCC TTCCAAGGAA  - #               30400                                                                         - - TGGGCTTCTG ATGCCTCTAC TGAGTGTGAT CTTCTTAAAT GGCAACTGTG  - #               30450                                                                         - - CCCGTGAAGA GGAAATCTGG GAATTCCTGA ATATGCTGGG GATCTATGAT  - #               30500                                                                         - - GGAAAGAGGC ACCTTATCTT TGGGGAACCC CGAAAGCTCA TCACCCAAGA  - #               30550                                                                         - - TCTGGTGCAG GAAAAATATC TGGAATACCA GCAGGTGCCC AACAGTGATC  - #               30600                                                                         - - CCCCACGCTA TCAATTCCTG TGGGGTCCAA GAGCTCATGC AGAAACCAGC  - #               30650                                                                         - - AAGATGAAAG TCCTGGAGTT TTTGGCCAAG GTGAATGACA CCACCCCCAA  - #               30700                                                                         - - TAACTTCCCA CTCCTTTATG AAGAGGCTTT GAGAGATGAA GAAGAGAGAG  - #               30750                                                                         - - CTGGAGCCCG GCCCAGAGTT GCAGCCAGGC GTGGCACTAC AGCCATGACT  - #               30800                                                                         - - AGTGCGTATT CCAGGGCCAC ATCCAGTAGC TCTTCCCAAC CCATGTGAGA  - #               30850                                                                         - - TCTAAGGCAA ATTGTTCACT TTGTGGTTGA AAGACCTGCT GCTTTCTCTG  - #               30900                                                                         - - TTCCTGTGAT GCATGAATAA CTCATTGATT TATCTCTTTG TTGTATTTTC  - #               30950                                                                         - - CATGATGTTT CTTAAAATAG AAAGTTTATT TAGATTCAGA ATATAAATTT  - #               31000                                                                         - - AGAAATGGCA TGCATCACAC ATTTATTGCT GTTTATCAGG TTGGTTTAGT  - #               31050                                                                         - - GATAATAATT TTGTTTTTGA AATACAAATA GAAAATCCTG AAATAATTTT  - #               31100                                                                         - - TGTGATACAG AGCAAAATAA CACGGCATGG GAGTAAGGTT ATCCTTAGAA  - #               31150                                                                         - - ATTTAAAATA ACTCCACAGT AAAATAGGTA GAATCTGAAG ATAGAAAGGG  - #               31200                                                                         - - AAGAAAAGTA AAAGTTGCTT TATTCGTGGT TTGTCTTACT CAGTTCAGTC  - #               31250                                                                         - - TTTTTTTGCT CATAAATTTA AAAGTTACAT ACCTGGTTTG CTTAGATTAT  - #               31300                                                                         - - TCAAGAATGT GGAGGCCTGG GCCAAGGTCA ATGACAGTGT CTCCATTGTC  - #               31350                                                                         - - TTCCCTCCAT TAAGAGAAGA CTTTAAGAGA TGAGGGAGAG AGAGCCAGAG  - #               31400                                                                         - - ACAGTGTTGC AACTGGGCCT GGCATGTTTC AGTGTGGTGT CCAGCAGTGT  - #               31450                                                                         - - CTCCCACTCC TTGTGAAGTC TGAGGTATAT TCTTTACTTT TGATTAAGAA  - #               31500                                                                         - - AACACTTAAC CTTCTAATTA ATGGAGAGCC AAAGGGGAGT TGGTGGGAAC  - #               31550                                                                         - - ACCATGTATA ACATATTTGT ATGTAAAATG ATTTATCTTT TCTTTTTCCT  - #               31600                                                                         - - GTTTTTCAGT GTTCTTTTTT TAAATTGTAG ATTTATTTAG TTTCAGAATC  - #               31650                                                                         - - TAAGTTTATG AATGGCATGA ATCACTCATT TATTAAAATA TATCAGGTTG  - #               31700                                                                         - - GAGAGTGAGA ATTTTTGCAT TATGTAAAAC AATTTAAAAA TCTTTTAAGT  - #               31750                                                                         - - CTTTTTCTGT GATCTAGAAC AAGATAATAT GGCATTGGAA TATGGAATTT  - #               31800                                                                         - - GTGAAAAGGA AATTACCTTG CAATAAAGTT GGTGGGACCA GGAAGTAGAG  - #               31850                                                                         - - AAAAAAAAAG TAAAATGTGG TCAATTCTTG CTTTGTTTTA TTCTTTTTAG  - #               31900                                                                         - - TCTTTCTTTG TAAAACTGAA GTATATGTAC CCGGATCTGC TTAGCTTTTT  - #               31950                                                                         - - CAGGAATGTG GGGGAAATTA AATAGCAATA CATTTGACTT CCTGGTCACT  - #               32000                                                                         - - TACACTTCAA TTGTCCAAAT ATTAATTGAG CAGCTGTACT TTGGAGGGCT  - #               32050                                                                         - - CCTGGCTAGT ACCGGATAAG CTAAGAAAAC AAAAAACAAA CAAACAACAA  - #               32100                                                                         - - AAAAACCCCA ATCCCTGCCC ACAGAATTTT AGAATCCAAC AGCAGCTATC  - #               32150                                                                         - - ATATAACGAA GGTAATGAGA TACTCCTTAA GACCTAAAGA CAGGTGAAAA  - #               32200                                                                         - - GGGGATAAAA AAGAAGGTGG GGGTATTACC AGCAGTGAAT CTAGTGTAAA  - #               32250                                                                         - - TGTCCTAAGC AAGGTAACTG AGACTTCGGG AAACTGAACA TACTGCAATA  - #               32300                                                                         - - GGAGGTAATT TTATGTCCTG GCATAGTGCC TATATGGGGC GCACTGAGCA  - #               32350                                                                         - - GGGGTCACAC ACTCAGATGG TGGATCTCAC ACTTGAGAGA TGGAGACTGG  - #               32400                                                                         - - AATGAAAAAT TGCCTTTAAC AGTTACTTTG GGATTGTGGA AAAACCAGAG  - #               32450                                                                         - - GGGATCTTTG CCTGGAGCAG GGATTGAAAG TGGCCGGTGC TCTTGTCCCA  - #               32500                                                                         - - GTGCAAGTGA AGTGAATCCA GTACACAACT AGGTGTTTAT GAGCGTAGTC  - #               32550                                                                         - - CCCAGGGTGT TCTGAGAAAT AAAGGTGATA CTCCTTTAAG ACTGGAAACT  - #               32600                                                                         - - CATAAGCCAC TGGGCTAATA CTCTGCTATC AGCTGGGGAG CCAGAACCCA  - #               32650                                                                         - - TTCCATTAAA AGGAATTTTT TTTCCTTTCA TGTGTTTTTG CAAATTCTAA  - #               32700                                                                         - - GGGAGATCTA GATTTTTGAT GGTGGTAAAA TGAATGAAAA TAAGACTGGT  - #               32750                                                                         - - TTGGATGAAA GGCCAGAGTT GGTCCTTGAA CCAAAGCCTA GAAGGTTTGA  - #               32800                                                                         - - GTTGCTTTCA GCTGAGAAAG ACAACTCCAC ACTCAAAATA TGATTTAATA  - #               32850                                                                         - - TGGAAAAGTT ATTTAGAGTT TCTTTGTAAG CTGCGTTTTG GGCTGATTTT  - #               32900                                                                         - - ATTGTTCCAT GTCTTAGAAT GCTGTATATT CTTTGTTATC TCTTGGACTT  - #               32950                                                                         - - AAAGAAAATC AGCAGGGTGG ATGATTTCTT CTGTAGTTGA AATAATCTTA  - #               33000                                                                         - - GTAATAACAA GTATTGTTTA AAGTCTCAAT GCACACCAGG GCTTTGCACA  - #               33050                                                                         - - CTTTCACCAC ATTCCACCAG TCCTACAAGA CAAGGCTTAT CAAGCTCATT  - #               33100                                                                         - - TCACAGATGT AGAGCCTGAG GCTCATAGAG TTTATTAATT GTGCCCAAGT  - #               33150                                                                         - - TCACACAGCT AGTAAGTGAT AGGGCTGGAA CTAGACTGCT GGTCTGAATT  - #               33200                                                                         - - CCTCTAGAGC CCACACTGTT CCACTCCTCC CAAACTGAAG CTTACTGTGA  - #               33250                                                                         - - ATTGCTTAAT TCATTTTCTT CTACAACCCC AAAGTGTGTC TCAGGTAAGA  - #               33300                                                                         - - AAGAAGAGGT CTCTGTACCC CAAACACTGG ATTGGAATAC ATAACCAGAG  - #               33350                                                                         - - CAAGAAGAAT ACCAAAATAA TTGACAGATG TGAATTAAAG AGAAAAAGAA  - #               33400                                                                         - - ATAATACTCG GTGACAATAT GATCACCGAC AGAAGCAAAA TTGGATAGCT  - #               33450                                                                         - - TCAAAAGATC AAAAGATTAC AAAAATCATC TGGCTCCACC CTTTTCTGTA  - #               33500                                                                         - - GAAAGTAAAA CTTTTAGAAC TACCCTTTGG TTGGTGAAGT GAAGGAATAG  - #               33550                                                                         - - ATCAGCATGT TTTTTTCCCT GAGAGCACAA CATCTGTCCT AGGCTCTCCA  - #               33600                                                                         - - CCTTCCGTTT CAGTTTCCCA ACATCACATC ACTTCTGGGA CAGGGACCTC  - #               33650                                                                         - - TTCTCTGCAA GGTTTGCAAA GAGGAAACTG GTCAAATTTT TGCAGGGATG  - #               33700                                                                         - - TGACATTTCC CTGGAAGAGT TTGATCAAGA GACAGAAGCC AACATGAGAA  - #               33750                                                                         - - CCCTCATGAA TGAATGGTGG GGAGAAAACC ACTCCAAAAT GTAAGGGTTC  - #               33800                                                                         - - ATCCAGACCC TCAGGAGAGT GACCTCAGTT CAGTAGATAG AGGAGTCCCA  - #               33850                                                                         - - GGTTCTGATA CATGTAAAGG CGAGGAGCAT AAATGAAGAC TGATGGAAGG  - #               33900                                                                         - - ACCCAGCCAA AACTGTGGGG TTCTATGGAG TCCTTTCCTT ATTATCTGTT  - #               33950                                                                         - - TTTGCAGGTT GCAAAAGGAC TGTGATCATA TGAAGATCAT CCAGGAGTAC  - #               34000                                                                         - - AACTCGAAAT TCTCAGAAAA CAGGACCTTG ATGTGAGAGG AGCAGGTTCA  - #               34050                                                                         - - GGTAAACAAA GGGTAAGTTA CAGGTTTGCT CACTTGTCAA GGTGAGGACC  - #               34100                                                                         - - TGAATGTGGA CTAAGGGTAG CTAGCACCCA CATGGCCTCA CAAAGTCCCC  - #               34150                                                                         - - TCTGCCTGTC AGCCCTAGGA AGCCTTGGCG AGATGGCAGG CTGAATTCTG  - #               34200                                                                         - - CCTGGAAAGT CTCAGGGAGA TGACTGGTTC CGTCTAATTG GGGCAGCCTC  - #               34250                                                                         - - AGTTTTACAG AGCGAAGAGG CCGAGACCCT AACAAGAATC AATGTAGGGC  - #               34300                                                                         - - TTTTAAGTGT TAAGAGGGGT ATCCACCAGC AGATGAGTCC CCACAGAATT  - #               34350                                                                         - - CACCCCGTTT TGAGGCATCA GACAGAGCTA TCTACCTAAG GTGCCTCTCA  - #               34400                                                                         - - TTTCCGCCTG GAAGGTCTCA TGGAGGATGG GGAGCGTGGG GCCTGAAGGG  - #               34450                                                                         - - AGTAGCTTCA GTTCTGCCTG GAGAGGAAAC CAGAGTCACG GTGAGGACTC  - #               34500                                                                         - - TGAGAGCTGA TGAGAAGGCC TCTGCCCAAA ACGGGACTTT CACAGAGCCC  - #               34550                                                                         - - TGCCGCTGCT GTCAGGCCTG TGAGGCCAGG CAGGGGTGGC CTGTGTGGCA  - #               34600                                                                         - - CGCTCAGATT TCCACCTTGG GGGCTGAGAG AGGTGGGGCT ATTGTTTGAG  - #               34650                                                                         - - GCTGGCGGAT TTGGGTCAGC AGGCGGAGTC GTCCCAGACT GCTAGATACT  - #               34700                                                                         - - AAGGTGAGGA CCCCTAGTGG GGACGTAGGG ACCAGCGACG CTAGAACAGT  - #               34750                                                                         - - TACGTCCAGA AGCGTACCAC CCCTGCCGTC AGCCCGGAGC CACGGGCTGC  - #               34800                                                                         - - CGGATGTGGC TCATCCTGAC TTCCGCTTTG AAGGCGAGGA CCCCAGCGAG  - #               34850                                                                         - - CGTAAGGGCG CAGTGTCCGC CTGGCGGATT TGGGTCAGCA GGCGGAAGTC  - #               34900                                                                         - - GTCCCAGGCT GCTAGATACT AAGGTGAGGA ACCCTAGTGG GGACGTAGGG  - #               34950                                                                         - - ACCAGCGACA CTAGAACAGT GACGTCCCGT AGCGTCCTGC CTCTGCCGTC  - #               35000                                                                         - - AGCCCTCAGA GGCCCTGGGC TGCCGGATGT GGCTCATCCT CATTTCCCTT  - #               35050                                                                         - - TTGAAGGCGA GGACCCGAGC GAGCTTAAGG AGTGGGGTGC AGCGTCTGGT  - #               35100                                                                         - - CAGCCGAGGG TGAATTCTCA GGACTGGTCG GGAGTCAAGG TGAGGACCCT  - #               35150                                                                         - - GAGTGTAAAT TGAAGAGACC ACCCCCACCC GTAACAAAGA GGTCCCCTCT  - #               35200                                                                         - - AAGTCCCGCT TCTGCATTTG GTCCTGGGAG GCCTCAGGTA ACCAGATGGG  - #               35250                                                                         - - TAGCACCCTG ACTGTCTCTT CAGCGACTCA GGGAGACGAA GGCTTTGGCC  - #               35300                                                                         - - TAAGCCTTAT AGACTCAGGT CAATAGAGGG AGGAGTCCTA AACCCTACTA  - #               35350                                                                         - - CCCGTAATCC CAGAACTCTG GGAGGCCGAG GCAGGCGGAT CACGAGGTCA  - #               35400                                                                         - - GGATATCAAG ACCATCCTGG CTAACACGGT GAAACCCCGT CTCTACTAAA  - #               35450                                                                         - - AATAGAAAAA ATTAGCCAGG TGTGGTGGTG GGCTGCTGTA GTCCCAGCTA  - #               35500                                                                         - - CTCAGGAGGC TGAGGCAGGA GAATGGCGTG AACCAGGGAA GCGGACGTTA  - #               35550                                                                         - - CAGTGAGCCG AGATTGCACC ACTGCACTCC AGCCTGGGTG ACAGAGCGAG  - #               35600                                                                         - - ACTCAGTCTC AAGAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAGTCCCGC  - #               35650                                                                         - - TCCTGCTGTC GGCACACGCA GGCCCCAGTC AGCCTTGGTG GGATGTGGCC  - #               35700                                                                         - - CACTATGACT GTGAACTTAG GTCCAAGGAA TATGAGAACT TTTGTCTACG  - #               35750                                                                         - - GGGCATGGTG TTAGGAGCAG TTGATGGGTG GAGTCCCAGA AAGAGTGCTG  - #               35800                                                                         - - AGTGGAGGTG GAGACACTGA GTGAGGAAAT GGCGGCTACT CTATACACGA  - #               35850                                                                         - - GGGAGACAAT TGCGCCTGAT GCTGTCCCTG GGAGTCCCAG GCCAGAGCTG  - #               35900                                                                         - - GCAGGCTCAA GTCTCCCTGG CTTCTGCCTT CATGGTCTTA GAGAGAGGAG  - #               35950                                                                         - - GGCCTTGGTT TAAGGCCTCA CTGCCCCAGT TCAGTAGAGA GATAGGAGTC  - #               36000                                                                         - - ATACGCCAAG ACAGGTGTCA AGGTACAATT TCTTTATGAG GAATGAGGTA  - #               36050                                                                         - - ACCGTCTAGT CCAGCAACAG GCAGGAACAC AGTCTTACCT TGGCGTTCAT  - #               36100                                                                         - - TCCTTAGAAG CCAGGGTTAG ACTCTCAAGT TGAGATGCCC CTCATTTCCT  - #               36150                                                                         - - TCAGGTACTG TTTCAGGTAC ATAATGGCTT TGGTTTAACA TGAAAGCCCT  - #               36200                                                                         - - TGTTTAGTAC AGGGAGGAGG CCCAGTTCCT AAAAGATGGT GACACTGAGG  - #               36250                                                                         - - GAGTATGAGG GGACCCCCTC CAAGAAAGAG GTAACACAGA TAGAGCCCTA  - #               36300                                                                         - - TCCCCACTGA GACCTGGGAG ATCCAGGTAT GGTGACATGA TGAGTCTCAC  - #               36350                                                                         - - TCACTTCTTC CTAGAGCATC TCAGGAAGTG GGAACTTCAT CAAAGGGGGC  - #               36400                                                                         - - AGCCTTATGT TAGCAGCTAG AGATTCCTAG TAATTCCAGG AGTCAAAAGA  - #               36450                                                                         - - AGACCCTGAG AACTGGGGAA CCACTCATCC CATAACAGTG AAAGAAACAC  - #               36500                                                                         - - GGAATTCCTT CCTGATTTTC ATCCTTGGGA GATGATGGAC AATTGTAGCC  - #               36550                                                                         - - AGATGGGAAA AGTTTCACTT CTTCCTCAGG GAGTTGTCGG GGGGTGCTTT  - #               36600                                                                         - - GTATGACTGG ATAGGCGTTA GATCAAGACA GAGAAGTCAC TGAGGGAGGA  - #               36650                                                                         - - ATGAGATGAG CATTTACCTA GAGAGTGGGC TTCACCAAGT TCTACCCCCT  - #               36700                                                                         - - CTCAGCTCTG GGAAACCCCA GGCAGAAGTA CCCAGATGTG TCATCCCCTC  - #               36750                                                                         - - AAGACTAGCC CTGGGTACTC AGAGAGGTGA AGGCTTTTGT ATGAGCCTGG  - #               36800                                                                         - - AAGAATCAAG TGGGATTAGA GAGGAGCTCA GACTCTGCTT GAGTGAAGAC  - #               36850                                                                         - - TAAAGAGAAC AACGACCCCA GAACAGTGGA GCCCCATAGA GGACGTGGTG  - #               36900                                                                         - - ACTGGATGTG ATTCAGGCTT TCTTTCATCT TGGGACTATG AGGCAATGAG  - #               36950                                                                         - - AATCTTAATC TGATGTAAGG GGCCTCAGGT CAGTAGAGAG AGGATTTCCA  - #               37000                                                                         - - GGTTGTGCCA GGCCTCATAG AGAGGACTTG AGGGAACTCC CACCTCATCA  - #               37050                                                                         - - GTGGGGATCC CTCAGAGTCC CTCTGTATGT CAGCGCTAGG AAGCCCCGAG  - #               37100                                                                         - - CATAAATGTC AGAAATGCCC CTAAATTCCT CTTCAGAAGT AACAGGGAAA  - #               37150                                                                         - - TGAAGGTCTT AGTCAGATGG GTTAGCAGGA GGGGTGGGAG GCATTTTAGG  - #               37200                                                                         - - CCCTCCCAGG AGTCAAACTG GAGACCTTGA GTGAGGACCA TGAGTGGGGC  - #               37250                                                                         - - TACACATCAC AGGGCTTCAA CCTGCCAGCC ACAGCTTTCA GCCTCTGGAG  - #               37300                                                                         - - AATATGGGCA TTGTGACCAG ATACAGCCAC CCTCATTTCC TCCATTGGGT  - #               37350                                                                         - - CTCAGGCAGA TGTAGGCCTT ACTATGAGTA AATGTCCTCA GGTGTAGAGA  - #               37400                                                                         - - GAACAGAGCC CTAGGCCCTT CTGGGAGTCG AAGTGTGAGT GTGGACTGAA  - #               37450                                                                         - - GGCACCATTC CCCCAAGCCC CCAACCCCCA CCCCAAATAG AGGAAAAACA  - #               37500                                                                         - - ACGATGCTAG CCCTGTCTCT GCACTTAGCT CTGAAAGGCC TTGGCCAAGG  - #               37550                                                                         - - GTTGCCAGGC TGAGACTTTA TTTCTTTGCA TCAGGTCTAA GGGAGGTGAC  - #               37600                                                                         - - AGCTTTGGTC TGAAGATGCA GCACCAGTTA GCAGAAGACA GGTTCCCAGA  - #               37650                                                                         - - ACTTAGATAT AGATGAGATG AGGACTCTGA ATTAAGATTG AGGTCCAACT  - #               37700                                                                         - - AGCCCAGGAC AGAGAGAGTT CCATAGAACT GTCAGCACTG CCATCCCGCC  - #               37750                                                                         - - AGCCCCCGGT AAGGATGGTA GGTTGAAGCA GTGCCTCATT TTTCTTTGTG  - #               37800                                                                         - - GATTCCAGGG AGCTTTGAAG TGTCAGCTTC AGAGCAGCAC AGGAAGGAGT  - #               37850                                                                         - - CCCAGACCCT TCCAAGAGTA GATATGAAGA TCCTGTATAT GAATTGAGAG  - #               37900                                                                         - - GCCTTGAACA CAGAGGAGTC TACACTGCCA ACCTCTGCTG TCACCCAGTC  - #               37950                                                                         - - AGCCCAGGCA GGTTTGGCAA CAAGAACCAG TGGTTCCTAG AGCAATGCCC  - #               38000                                                                         - - TCAAGAAAAC CAGCAGAAGT GCTCTCTAAA AGCCAAGTTG TACCTCCCTG  - #               38050                                                                         - - CTGCAAGTAC TCACAGATCT CATTCTCTCT CCTTCAGGTG CCACATCTCC  - #               38100                                                                         - - TGCCTTTCTG CTCACTTTCC TGCCTGTTTT GCCTGACCAC AGCCATCATG  - #               38150                                                                         - - CCTCGGGGTC AGAAGAGTAA GCTCCGTGCT CGTGAGAAAC GCCGCAAGGC  - #               38200                                                                         - - GCGAGAGGAG ACCCAGGGTC TCAAGGTTCG TCACGCCACT GCAGCAGAGA  - #               38250                                                                         - - AAGAGGAGTG CCCCTCCTCC TCTCCTGTTT TAGGGGATAC TCCCACAAGC  - #               38300                                                                         - - TCCCCTGCTG CTGGCATTCC CCAGAAGCCT CAGGGAGCTC CACCCACCAC  - #               38350                                                                         - - CACTGCTGCT GCAGCTGTGT CATGTACCGA ATCTGACGAA GGTGCCAAAT  - #               38400                                                                         - - GCCAAGGTGA GGAAAATGCA AGTTTCTCCC AGGCCACAAC ATCCACTGAG  - #               38450                                                                         - - AGCTCAGTCA AAGATCCTGT AGCCTGGGAG GCAGGAATGC TGATGCACTT  - #               38500                                                                         - - CATTCTACGT AAGTATAAAA TGAGAGAGCC CATTATGAAG GCAGATATGC  - #               38550                                                                         - - TGAAGGTTGT TGATGAAAAG TACAAGGATC ACTTCACTGA GATCCTCAAT  - #               38600                                                                         - - GGAGCCTCTC GCCGCTTGGA GCTCGTCTTT GGCCTTGATT TGAAGGAAGA  - #               38650                                                                         - - CAACCCTAGT AGCCACACCT ACACCCTCGT CAGTAAGCTA AACCTCACCA  - #               38700                                                                         - - ATGATGGAAA CCTGAGCAAT GATTGGGACT TTCCCAGGAA TGGGCTTCTG  - #               38750                                                                         - - ATGCCTCTCC TGGGTGTGAT CTTCTTAAAG GGCAACTCTG CCACCGAGGA  - #               38800                                                                         - - AGAGATCTGG AAATTCATGA ATGTGTTGGG AGCCTATGAT GGAGAGGAGC  - #               38850                                                                         - - ACTTAATCTA TGGGGAACCC CGTAAGTTCA TCACCCAAGA TCTGGTGCAG  - #               38900                                                                         - - GAAAAATATC TGAAGTACGA GCAGGTGCCC AACAGTGATC CCCCACGCTA  - #               38950                                                                         - - TCAATTCCTA TGGGGTCCGA GAGCCTATGC TGAAACCACC AAGATGAAAG  - #               39000                                                                         - - TCCTCGAGTT TTTGGCCAAG ATGAATGGTG CCACTCCCCG TGACTTCCCA  - #               39050                                                                         - - TCCCATTATG AAGAGGCTTT GAGAGATGAG GAAGAGAGAG CCCAAGTCCG  - #               39100                                                                         - - ATCCAGTGTT AGAGCCAGGC GTCGCACTAC TGCCACGACT TTTAGAGCGC  - #               39150                                                                         - - GTTCTAGAGC CCCATTCAGC AGGTCCTCCC ACCCCATGTG AGAACTCAGG  - #               39200                                                                         - - CAGATTGTTC ACTTTGTTTT TGTGGCAAGA TGCCAACCTT TTGAAGTAGT  - #               39250                                                                         - - GAGCAGCCAA GATATGGCTA GAGAGATCAT CATATATATC TCCTTTGTGT  - #               39300                                                                         - - TCCTGTTAAA CATTAGTATC TTTCAAGTGT TTTTCTTTTA ATAGAATGTT  - #               39350                                                                         - - TATTTAGAGT TGGGATCTAT GTCTATGAGC GACATGGATC ACACATTTAT  - #               39400                                                                         - - TGGTGCTGCC AGCTTTAAGC ATAAGAGTTT TGATATTCTA TATTTTTCAA  - #               39450                                                                         - - ATCCTTGAAT CTTTTTTGGG TTGAAGAAGA AGAAAGCATA GCTTTAGAAT  - #               39500                                                                         - - AGAGATTTTC TCAGAAATGT GTGAAAGAAC CTCACACAAC ATAATTGGAG  - #               39550                                                                         - - TCTTAAAATA GAGGAAGAGT AAGCAAAGCA TGTCAAGTTT TTGTTTTCTG  - #               39600                                                                         - - CATTCAGTTT TGTTTTTGTA AAATCCAAAG ATACATACCT GGTTGTTTTT  - #               39650                                                                         - - AGCCTTTTCA AGAATGCAGA TAAAATAAAT AGTAATAAAT TATATTACTT  - #               39700                                                                         - - GTTCAGTGGC TCATTTATTC TCACCATAAA TTGAGCATCT GCTCTTTGTA  - #               39750                                                                         - - AGGCTCTGTG ATAGTAGTGA TTGTACTAAG TTAAAGAAGA CCCTTCGCCT  - #               39800                                                                         - - GCACACAGAT TTTTAGTCTA AGGACAGTTA TTATTTAAAG AAGATGGTGA  - #               39850                                                                         - - GATACACTCT AACATGTACA GATTTTTTTT TTTACATATA AACACTCATT  - #               39900                                                                         - - TAAAAAAAAA AGAAGTGAGA ATGGTGGGAG AAGGTTCAGA CAAGAGCAGT  - #               39950                                                                         - - CAAGTGTTAA TTTCCTAGCC AAGGCACTTC GTGGTGTGGG ACAATGCAAG  - #               40000                                                                         - - TCCCTCGTTG GGAGGTCATT TTAAGTTAGC TCCATGGTGA ACTGGATGAG  - #               40050                                                                         - - GTTGTGATAA TCATAAGAAG GTGCCAAACC CTCAGATCAT GAGCCTTAGA  - #               40100                                                                         - - GTTGAGAGAT TAAGCCTGGA AAGGGAAACT GCCCTTAACA GTTACTTTGG  - #               40150                                                                         - - GATTGTGGGT AAAGCAGAGA GAACCTGTGT CTGGAGGAGG AGTGGAAGAA  - #               40200                                                                         - - TACAGTGCTC TCGTCCCAGG GCAGTCAAAC ACAGTGCACA AACTAGTTGT  - #               40250                                                                         - - TTTATGCACA TTGTCTCCAG AAAGTGTTTG AGAAATAAGG GTTATACTTC  - #               40300                                                                         - - CTTGAGGTGA GATGCCAAGA AGCCACTAAG CTAACACTGT TTCCCTAAGC  - #               40350                                                                         - - TT                  - #                  - #                  - #               40352                                                                   - -  - - (2) INFORMATION FOR SEQ ID NO: 16:                                   - -      (i) SEQUENCE CHARACTERISTICS:                                                 (A) LENGTH:   23 - #base pairs                                                (B) TYPE:  nucleic a - #cid                                                   (C) STRANDEDNESS:  sing - #le                                                 (D) TOPOLOGY:  linear                                                - -     (xi) SEQUENCE DESCRIPTION:  SEQ ID NO: - # 16:                        - - GATCATCCAG GAGTACAACT CGA           - #                  - #                    23                                                                      - -  - - (2) INFORMATION FOR SEQ ID NO: 17:                                   - -      (i) SEQUENCE CHARACTERISTICS:                                                 (A) LENGTH:   20 - #base pairs                                                (B) TYPE:  nucleic a - #cid                                                   (C) STRANDEDNESS:  sing - #le                                                 (D) TOPOLOGY:  linear                                                - -     (xi) SEQUENCE DESCRIPTION:  SEQ ID NO: - # 17:                        - - CCCGAGCGAG CTTAAGGAGT            - #                  - #                      - # 20                                                                   - -  - - (2) INFORMATION FOR SEQ ID NO: 18:                                   - -      (i) SEQUENCE CHARACTERISTICS:                                                 (A) LENGTH:   19 - #base pairs                                                (B) TYPE:  nucleic a - #cid                                                   (C) STRANDEDNESS:  sing - #le                                                 (D) TOPOLOGY:  linear                                                - -     (xi) SEQUENCE DESCRIPTION:  SEQ ID NO: - # 18:                        - - AGCGAGTGTA GGGGGTGCG             - #                  - #                      - # 19                                                                 __________________________________________________________________________

We claim:
 1. Isolated nucleic acid molecule, consisting of nucleotides3266-7791 of SEQ ID NO:15, nucleotides 23545-25193 of SEQ ID NO:15,nucleotides 29747-31473 of SEQ ID NO:15, nucleotides 29747-31827 of SEQID NO: 15, or nucleotides of 31402-39690 of SEQ ID NO:15.
 2. Method forscreening for possible presence of lymphoma, or head and neck squamouscell carcinoma, in a sample, comprising assaying said sample for mRNAfor MAGE-B2, wherein presence of said mRNA is indicative of possiblepresence of lymphoma or head and neck squamous cell carcinoma in saidsample.
 3. The method of claim 2, comprising determining said mRNA bymeans of a polymerase chain reaction which comprises using theoligonucleotides set forth in SEQ ID NO:5 and SEQ ID NO:6, or SEQ IDNO:18 and SEQ ID NO:6 as primers.
 4. Isolated nucleic acid moleculeuseful as an obligonucleotide primer, said isolated nucleic acidmolecule having a nucleotide sequence as set forth inSEQ ID NO:16, SEQID NO:17, or SEQ ID NO:18.
 5. Kit useful in amplifying a MAGE-B gene,comprising a pair of oligonucleotide primers, said pair being selectedfrom the group consisting of: (a) SEQ ID NO:12 and one of SEQ ID NO:11,16 or 17 and (b) SEQ ID NO:6 and SEQ ID NO:18.
 6. Method for screeningfor possible presence of skin carcinoma or mammary carcinoma, comprisingassaying a sample for presence of mRNA for MAGE-B1, wherein said mRNAcomprises a transcript of at least exons 3 and 4 of a MAGE-B1 gene, viaa polymerase chain reaction carried out using the oligonucleotide setforth in SEQ ID NO:12 as a primer, and one of the oligonucleotides setforth in SEQ ID NO:11, SEQ ID NO:16, or SEQ ID NO:17 as a primer,wherein presence of said mRNA is indicative of possible presence of shincarcinoma or mammary carcinoma in said sample.
 7. Isolated nucleic acidmolecule useful as an oligonucleotide primer, said isolated nucleic acidmolecule having the nucleotide sequence set forth inSEQ ID NO:12. 8.Method for screening for possible presence of leukemia or mammarycarcinoma in a sample, comprising assaying said sample for mRNA forMAGE-B2 by contacting said sample with a pair of oligonucleotides, thenucleotide sequences of which are set forth in SEQ ID NO:6 and SEQ IDNO:18, and determining hybridization of said pair of oligonucleotides tosaid mRNA for MAGE-B2, said hybridization being indicative of possiblepresence of leukemia or mammary carcinoma.